降纤酶对脊髓损伤后血管内皮生长因子的表达及细胞凋亡的影响-外科学(骨外)专业论文.docxVIP

Defibrase Defibrase influence to the expression of VEGF and apoptosis after spinal cord injury PAGE PAGE 6 time points after 3 days(P0.05),among which the score of the two groups was apparently different at 7,14,28 days time pionts(P0.01).The result of VEGF IHC: except that a few VEGF expressed around central canal and soft meninges of spinal cord, it seldomly expressed in other locations in sham group; In control group, after spinal cord injury VEGF highly expressed in the injured area and its periphery, which reached the peak at 5d，decreased after 5d，obviously expressed at 7d、14d and still expressed at 28d. While in intervention group, the expression of VEGF significantly increased at 1d、3d、5d、7d、14d, 28d compared to control group, and the difference was significant (P0.05), among which the expression of VEGF between the two groups were apparently different at 3,5,7,14days time pionts(P0.01). The result of TUNEL labeled cell apoptosis: a little TUNEL labeled positive cells were observed at every time point in sham group, while those cells could be observed obviously after spinal cord injury in the control and intervention groups.Apoptotic cells gradually increased after injury, reached the peak at 1d, decreased after that. At 5d, 7d time points there were still many apoptotic cells and still existed at 14d, 28d. Those positive cells mainly existed in neurons and astrocytes, the apoptosis positive cell in intervention group was significantly lower at 1d, 3d, 5d, 7d ,14d, 28d when compared to control group, which was significantly different(P 0.05), among which it was apparently different at 3d,5d,7d time pionts(P0.01). The correlation between VEGF positive cells and apoptosis positive cells at the time point of 1d、3d、5d in pure injured group was analyzed, and the result was that the expression of VEGF was negatively correlate with the positive apoptosis cells at these time points (correlation index r = -0.90052, P 0.05）. Conclusions ①The expression of VEGF afte