绞股蓝皂苷对HepG2细胞的凋亡、转移侵袭的影响-食品科学专业论文.docxVIP

ABSTRACTIn ABSTRACT In recent years，people ale looking for new natural medicines inhibiting tumor occurrence and metastasis．Saponins are widely distributed in the plant kingdom，and have good antitumor effect．Saponins could not only inhibit the proliferation of cancer，but also enhance immunity，and inhibit tumor metastasis．Gynostemma Pentaphyllum(GP)belongs to the medicine food homology plant，and gypenosides are the main active ingredient．In the aspect of anti-cancer,there have been many studies about inhibition of tumor cell of the total saponins extracted from GP at present，however,the monomer gypenosides are still rarely reported and their underlying molecular mechanisms have not been well defined． This subject studied the mechanism of induction cancer cells apoptosis，inhibiting metastasis of three monomer gypenosides N-2，J-0—1，J-D-40 by applying different assays． In this study，inhibition of human hepatocarcinoma HepG2 cells proliferation was determined by MTT assay；Scanning electronic microscopy(SEM)to observe changes in cell morphology；nuclear morphology by DAPI staining was observed witll fluorescence microscope；Apoptosis Was assessed by flow cytometry after staining the cells witll annexin V-FITC；Mitochondrial transmembrane potential was detected by Rhodamine-123 staining；Experiments of wound healing and Transwell were used to detect the ability of cell migration，cell invas．ion．The expression of p53，MMP-2，MMP-9，and HIF—l a mRNA by RT-PCR and proteins by Western blotting were also performed． The results show that gypenosides N一2，J-0-1，J-D-40 have the ability to induce cell apoptosis and inhibit metastasis．Samples inhibited HepG2 cells proliferation，and the inhibition Was dose—dependent；After treatment with ginsenoside R93 200 lunol／L，N-2 200 lxrnol／L，J-0-1 1 00 lxrnol／L，J-D-40 50 lamol／L，the HepG2 cells occured typical apoptosis morphology．Samples increased the number of apoptosis cells，elevated the mitochondrial membrane potential．The ability