结肠癌细胞上皮间质转化后β1整合素表达变化及意义-普外科专业论文.docxVIP

万方数据 万方数据 The effect of epithelial-mesenchymal transition of human colon carcinoma on integrin-β1 expression and significance Abstract Objective: To investigate the expression of integrin-β1 of human colon carcinoma cell line SW480 after epithelial-mesenchymal transition(EMT) and effect of invasive ability on the human colon carcinoma in vitro. Methods：TGF-β1 (10ng/ml) was used to introduce EMT of SW480 cells for 72 hours . Real-time PCR and Western-blot were used to detect expression of intergin-β1 of SW480 cell. The migration of SW480 cell in vitro was assayed by transwell. Result：In contrast with control group, the expression of integrin-β1 mRNA of EMT group was increased obviously （ t=3.764,P0.05 ） . Comparing with control group （ integrin- β 1/ β -actin=0.172±0.034）, the expression of integrin-β1 protein of SW480 cell of EMT group （integrin-β1/β-actin=0.430±0.021）was increased obviously（P0.05）. In transwell, the number of migration of SW480 cell in EMT group（137±9）was significantly more than that in control group （36±4）（P＜0.05）. Conciusion：The expression of integrin-β1 and the migration of human colon carcinoma cell in vitro can be elevated after EMT. Master student：JI Yishun（General Surgery） Directed by Prof. Gao Jun KEY WORDS: Colon carcinoma；Epithelial-mesenchymal transition；integrin-β1 目 录 第一章 引言 ·······························································································································1 第二章 结肠癌细胞的培养和上皮间质转化细胞的构建····················································3 2.1 主要仪器和试剂 ···········································································································3 2.2 液体配置 ·························································································································3 2.3 细胞培养 ··························································································································3 2.4 上皮间质转化细胞的构建 ·················