降钙素对兔膝关节骨关节炎关节软骨的保护作用-外科学专业论文.docxVIP

中 中 文 摘 要 PAGE PAGE 10 健侧 P0.01 进一步说明了大白兔膝关节 ACLT 后 6 周骨关节 炎造模成功 2)对照组手术膝关节ACLT+NS 组和实验组手术膝关节 ACLT+CT 组 对比结果 ACLT+CT 组膝关节大体观察可见关 节面粗糙 颜色发暗 未见胫骨平台骨性肥大 关节边缘仅有少 量骨赘形成 关节软骨损害较 ACLT+NS 组轻 两组膝关节大体评分和组织学评分 番红 O 染色和型 胶原免疫组化染色平均灰度值测量结果对比 ACLT+CT 组也明 显低于 ACLT+NS 组 P0.05 说明使用降钙素明显减轻了关节 软骨的退变 阻止了关节基质成分的破坏和丢失 结论 1 本实验证实 采用 ACLT 方法对大白兔膝关节骨关节炎造 模确实可靠 术后 6 周骨关节炎造模成功 2 降钙素 5 IU/kg 每日一次皮下注射能够明显减轻兔膝关节 不稳定诱发的骨关节炎关节软骨的退变 3 降钙素可能通过提高软骨基质 GAG 和型胶原含量保护 关节软骨 关键词 降钙素 骨关节炎 关节软骨 灰度值 ACLT 英 英 文 摘 要縮寫 PROTECTION OF CALCITONIN ON ARTICULAR CARTILAGE OF RABBIT KNEE JOINT WITH OSTEOARTHRITIS Abstract Objective: To make osteoarthritis model by anterior cruciate ligament transection (ACLT) ;To assess whether calcitonin exerts a chondroprotective action in unstable knees which induce experimental osteoarthritis . Methods: twenty 6-month New Zealand rabbits were divided randomly into two groups. All rabbits underwent anterior cruciate ligament transection (ACLT) on the right knee. Rabbits of experimental group began to receive a daily subcutaneous injection of salmon calcitonin at a dose of 5 IU per kg of body weight on day 7 postsurgery for 5 weeks; In contrast ,control group received NS(normal saline) at the same dose. All rabbits were killed on day 42 postsurgery. The knee joints were examined grossly and histologically and scored; The medial condyle sections were stained with Safranin O and immunohistochemistry of collagen . we applied a computer graphic technology to assess the glycosaminoglycans (GAG) and collagen of articular cartilage. Results: 1) In control group, compare uninjured knee joints and operative knee joints : Surfaces of uninjured knee joints were all smoothy and glossy, displayed a little light blue; synovial was clear and transparent; Operative knee joints all swelled, their surfaces were uneven or anabrotic, displayed dim and bloomy; synovial was light yellow and cloudy, their tibial plateaus appeared hypertrophic, oste