结香花挥发油的气相色谱—质谱联用分析-植物学(植物化学)专业论文.docxVIP

摘 摘 要 本论文还对重要药用植物瑞香科(Thymelaeaceae)结香属(Edgeworthia)植 物结香(Edgeworthia Chrysarntha Lind]．)的鲜花的挥发油进行了气相色谱一质 谱联用法分析。使用乙醚萃取浓缩、水蒸气蒸馏的方法提取获得结香花挥发油 利用气相色谱一质谱联用法分离并分析其化学成分，采用Gc峰面积归一化法定 量。色谱条件：石英毛细管柱：HP一5(30 nl×0．25 m×0．25 m)，载气为 氦气，流速为1 mL／min。程序升温从30℃(保留5分钟)开始以8C／min升到 120℃，辑20℃／min，升至240℃，保留10 min。质谱条件：电离源为EI 电离能量70 ev，离子源温度230℃．扫描范围：30—460 u。结果鉴定出的 成分有62种，共占挥发油总量的60．14％。其主要成分涵盖了烷，烯，芳香环 酮，醚，酚，醇，醛，酸和酯类等，它们中的绝大部分都具有较大的化工和医药 价值。其特有成分有1个含氮化合物、癸醚、7一溴甲基一7一十五烯和十六烷叔硫 醇等。 实验证明用气相色谱一质谱联用分析挥发油化学成分的方法稳定可靠，重现 性好，适用于结香花挥发油的化学成分分析。同时，本论就与文献报道的用固相 微萃取SPME技术提取的不同香气化学成分做了简单的比较，综述了此植物的化 学成分及它的次生代谢产物在化学生态学中的地位，为进一步地开发和利用该植 物提供必要的实验和理论依据。 [关键词]结香鲜花； 挥发油； 化学成分： 气相色谱一质谱联用 Il AbstractThe Abstract The chemical components of essential oil from the flowers of Edgeworthia chrysantha Lindl．of Edgeworthia of ThymeIaeaceae analyzed by GC—MS in this paper．The volatile components extracted by diethyl ether，then isolated by steam dJstillation method．Their chemical compositions and relative contents analyzed using GC—MS with the condition of chromatogram of HP一5 5％phenyl methyl siloxane capillary column(30 m×0．25 mm X 0．25№)．The carrying gas helium， the velocity of flow was 1 mL／min and the temperature was in rate of 8 ℃／min from 30℃(hold 5 min)to 120℃，then in rate of 20℃／min from 120。C to 240。C(kept 10 min)．And with the condition of mass spectrum of El ionization，the energy of ionization WaS 70 eV，the ionization temperature was 230℃．and the spectrum range of the scan was 30—460 u．As a result．sixty—two volatile compounds of Flos Edgeworthlae were identified，which comprised 60．14％of volatile fraction．The main components include alkylv，alkene，aromatic ring、ketone、aether、 hydroxybenzene、mellow、aldehyde、acid and ester，which have good value in industry of chemistry and medicine One nitrogenous configurations decane，l，1’一oxybis一：7-pentadecene7一bromomethyl—and tert—Hexadecane一 一thiol were especial components Our results indecate the analytical method of GC—MS is reliable stabilize and repeatability，therefore t