金福菇多糖抗衰老作用研究-细胞生物学专业论文.docxVIP

AbstractAging Abstract Aging is a long and gradual process that the physiological functions decline gradually in living organisms and they will die at the end．In recent years，the problem of agmg has been becoming increasingly serious and more and more people have focus on the research on alleviating aging and the mechanism of anti-aging．In order to reduce the oxidative damage to the organism of free radicals，a lot of synthetic antioxidants have been widely used．Tricholoma lobayense，a kind of nutrition-rich and valuable edible fungus distributed in tropical and subtropical regions，possesses many kinds of biological activities．In recent years，a lot of researches have shown that synthetic antioxidants should be restricted due to their potential hazards related to organism health．In view of the potent danger,it is becoming increasingly important to search safe and non．toxic antioxidants from natural products to substitute the synthetic antioxidants．The researchers in our laboratory have focused on Tricholoma lobayense polysaccharides and their various biological activities in recent years．In our previous studX Tricholoma lobayense polysaccharide TLH-3 possessed very excellent antioxidant activity／n vitro experiment and its ability to scavenge the DPPH radical Was comparable to that of Vc with IC50 value of 1 26 p．g／mL．Tricholoma lobayense polysaccharide TLH一3 also Can reduce the content of MDA．Besides，it possesses the ability to protect the HELF cells injured by tert-butylhydroperoxide (t-BI-IP)．Tricholoma lobayense polysaccharides TLH and TLH一3 call improve the antioxidant capacity of aging mice induced by D-gal and retard the aging process highly effectively．Therefore，to investigate the anti-aging bioactivity of TLH and TLH-3 further,the present study was conducted to determine the anti·aging activity of Tricholoma lobayense polysaccharides／n vitro by t-BHP induced HELF ceils damage and in aged mice induced by D-gal．The research provides a theoretical basis for the na