HIV抗体不确定结果剖析.DOC

HIV 抗体确证试验不确定结果的解析 崔海燕1 ，薛秀娟2 ，刘春华2，张倩1 ，李明1 ， 李颖1， 徐涛1 驻马店市疾病预防控制中心，河南 驻马店 463000； 2. 河南省疾病预防控制中心 性病艾滋病防治研究所，郑州 450016 ) 摘要：目的 分析艾滋病病毒（HIV）抗体不确定者的检测结果 ，探讨HIV抗体不确定结果出现的规律和HIV不确定人群蛋白印记试验（WB）带型的变化特征。方法 对驻马店市HIV确证实验室2013-2015年报告的55例HIV抗体不确定人群进行跟踪随访，回顾性研究其WB带型及HIV酶联免疫吸附试验（ELISA）的S/CO值特征，观察其最终临床转归过程中的发展和变化。 结果 2013-2015年驻马店地区共检测HIV抗体不确定者55例。经随访检测，有30例（54.5%）确证为阳性，21例（38.2%）确证阴性，4例 (7.3%)失访。WB条带的组合模式共有18种。单次带型分析显示，p24带型出现26次，占总条带数的23.0%；其次是gp160，占总带数的19.5%。HIV筛查试验ELISA的不同S/CO值的样本阳转率差异有统计学意义（?2=16.259，P=0.006）。 结论 驻马店市近几年发现HIV不确定结果中，env带的出现及HIV筛查试验ELISA的S/CO＞6.0预示HIV感染的可能性较大，对HIV感染的判断应结合流行病学史及实验室相关检测手段进行综合诊断。 关键词： 抗体不确定；蛋白印迹试验带型；酶联免疫吸附试验 中图分类号：R512.91；R373.9　　文献标志码：A 文章编号: 1672-5662(2017)00-0000-00 To analyze the uncertainty results of HIV antibody was confirmed by Western blot Cui Haiyan1, Xue Xiujuan2, Liu Chunhua2, Zhang Qian1, Li Ming Li Ying1,Xu Tao. (1.Center for AIDS/STD Control and Prevention, Zhumadian Center for Disease Control and Prevention，Zhumadian 463000，China； 2. Center for AIDS/STD Control and Prevention, Henan Center for Disease Control and Prevention) Corresponding author：Xue Xiujuan， Email： HYPERLINK mailto:boboo2009@126.com boboo2009@126.com Supported by Basic and advanced technology research of Henan (162300410123) and Medical science and technology research projects in Henan Province(201602311) Abstract: objective Through the analysis of the uncertainty results of HIV antibody detection, the regularity of HIV antibody uncertainty and the variation of Western blot(WB) band pattern in HIV uncertain population were discussed. Methods 55 cases of HIV antibody uncertain people were followed up in HIV-positive laboratories in Zhumadian City from 2013 to2015. To observe the development and changes in the final clinical outcome of the people, the characteristics of S/CO of Enzyme-linked immunosorbent assay(ELISA) and WB band were studied retrospectively. Results 55 cases of HIV antibody uncertainty were detecte