表观遗传药物对体细胞核移植胚胎发育的促进作用.docxVIP

表观遗传药物对体细胞核移植胚胎发育 的促进作用 盼盼杨树宝栾维民马馨 吉林农业大学动物科学技术学院 摘要： 体细胞核移植(somatic cell nuclcar transfer, SCNT)是利用卵母细胞胞质 中的重编程物质对高度分化体细胞核进行重编程作用使其恢复全能性并发育为 新个体的技术。在SCNT过程中，表观遗传修饰参与卵母细胞的重编程，如DNA 甲基化修饰和组蛋白的翻译后修饰。这些重编程的异常修饰会对SCNT胚胎的发 育产生不良影响。表观遗传药物，如DNA甲基转移酶抑制剂和组蛋白去乙酰基酶 抑制剂，可改善表观遗传修饰的异常现象，促进体细胞核移植重构胚的重编程。 该文对SCNT胚胎重编程过程中的异常表观遗传修饰以及近年来报道的表观遗传 相关药物进行综述，并进一步探讨了这些药物对SCNT胚胎发育的促进作用。 关键词： 表观遗传药物;体细胞核移植;重编程;胚胎; 作者简介：栾维民 Tel:0431-8453480;E-ma订：lua.nwciminl957@163. com; 作者简介：马馨 Tel:0431-8453812, E-mail:maxin3202@163. com 收稿日期：2017-05-26 基金:吉林省自然科学基金(批准号:20170101018JC) Progress on Epigenetic Drugs in Development of Somatic Cell Nuclear Transfer Embryos Ma Panpan Yang Shubao Luan Weimin Ma Xin College ofAnimal Science and Technology, Jilin Agricultural University; Abstract： Somatic cell nuclear transfer (SCNT) is a technology, which makes reprogramming of highly differentiated somatic cell nuclear by making use of cytoplasmic reprogramming material in the oocyte, finally restores the totipotency and develops into a new number. Tn SCNT, epigenetic modifiedtions participate in reprogramming of oocyte, such as modifications of DNA methylation patterns and his tone modif ica tion after translation, their abnormal expression has adverse impacts on the development of SCNT embryos. Epigenetic drugs, such as DNA methyItremsferase inhibitors and histone deacetylase inhibitors, can improve the abnonual cpigcnctic modifications and promote the reprogramming of SCNT embryos. This paper summarizes the abnormal epigenetic modifications in the process of SCNT embryonic development as well as the progress of epigenetic drugs. The effects of the drugs on SCNT embryos development were discussed further. Keyword： epigenetic drugs; SCNT; reprogramming; embryos; Received： 2017-05-26 体细胞核移植(somatic cell nuelear transfer, SCNT技术已成功应用于家畜 繁殖、转基因动物生产和濒危物种保护等领域。牛是SCNT技术研究和应用最广 泛的物种，已有良种公牛克隆、高泌乳性能奶牛培育和濒危物种成功保护等报道, 然而SCNT的牛胚胎发育率以及胚胎移植后的出生率普遍较低4。这主耍是由于 SCNT后，基因组进行了重要的表观遗传修饰，如DNA去甲基化和组蛋白修饰， 这些修饰对新形成的受精卵和随后的胚胎发育至关重要R1，但在SCNT