海水养殖鱼类淋巴囊肿病的快速检测-水产养殖专业论文.docxVIP

2000青岛海洋大学硕士毕业论文器官，率先入侵其脾脏，一旦吞噬细胞不能完全消灭病毒时，则会相 2000青岛海洋大学硕士毕业论文 器官，率先入侵其脾脏，一旦吞噬细胞不能完全消灭病毒时，则会相 继入侵其他内脏器官。 从濒死的大菱鲆体内分离到四株细菌，通过药敏试验发现，这四 株细菌对氟哌酸、氯霉素等药物敏感。至于这四株菌是否为同一种细 菌尚需进一步分类鉴定。组织病理学显示大菱鲆肾脏的病变是其死亡 ＼ 的主要原因。4、 关键词： 牙鲆、眼斑拟石首督黑硝大菱鲆淋巴囊肿硝虹彩病、 毒 酶联免疫吸附反应。免疫荧光抗体√病理组织学 兰———————————苎型坐!坚!竺!查竺!主!竺竺查Abstract 兰———————————苎型坐!坚!竺!查竺!主!竺竺查 Abstract In the paper it is deliberated the methods that Durify lymphocystis diseases virus(LCDV)of Japanese Flounder and that inspect the disease fast，made research on the route of LCDV infect ing Japanese flounder、 red drum、perch and dark rockfi sh，analyzed the reason of turbot’s death from the viewpoint of pathotogy，and made out the relevant tactics of prevent ion at the meanwhi le． The clinical symptom of Lymphocystis diseases is characterised by whitish nodules，which are aggregated of hypertrophic cells，scattered on the fins、backskin、mouth and orbit of affacted fishes．Most of these nodules are white，or slight grey，or haematinus．The nodules，whose sizes are different from imm to even more than 20mm in diameter，are Dacked tightly together just tike moruloid or tophaceous．Electron ultramicroscopics of nodules of Japanese flounder，red drum and dark rockfish show that the virions with hexagonal envelope are measured about 260nm，220nm and 2lOnm from corner to corner， respectively．Histopathological sections show that the enlarged cells of Japanese flounder are filled with the virion inclusion body， which lies along the elastic and thick cell wall 1ike floccule，the nucleus is spewed to deviate from the centre of the enlarged cell by the virion inclusion body．While the virion inclusion body of red drum and dark rockfish lies along the thicker cell wall 1ike a slim chain． Counter immuno— electrophoresiS show that the virus from three kinds of fisbes haye reaction with Japanese flounder anti——LCDV immunization ——． ——． !竺!Q!苎!!兰查竺!查!!竺查 一5 serum·Feulgen’S stain shows that the virus belongs to DNA Virus． The virus purified by sucro