灌胃“益气养阴方”大鼠的血清诱导白血病K562细胞凋亡的实验分析-中西医结合基础专业论文.docxVIP

占01163提要 占01163 提要 目的：探讨“益气养阴方”治疗白血病的作用机理。方法：运用血清药理学方 。●__----_-。一 法孵育人白血病细胞系K562细胞，通过荧光光度测定法、流式细胞术、免疫组化法 分别检测灌胃大、中、小剂量的“益气养阴方”大鼠的血清对膜流动性、细胞周期 和细胞凋亡及bcl一2、突变型p53基因表达的影响。／结果：小剂量组对细胞膜流动 L 性无明显影响。而大剂量组使K562细胞膜流动性增高，表现出与注射砒霜大鼠的血 清和灌胃羟媾脲大鼠的血清相同的趋势：灌胃三利哜4量“益气养阴方”大鼠的血清 均能使K562细胞s期细胞百分数增加，Go—GI期细胞百分数减少，与注射砒霜大鼠 的血清影响结果相似。三种剂量的血清均能使K562细胞bcl-2和突变型p53基因表 达减少，它们都能诱导K562细胞调亡。厂结论：灌胃大、-I-、小剂量“益气养阴方” 大鼠的血清均能使K562细胞阻滞予s期．并且诱导K562细胞调亡。这种作用可能 与其使K562细胞膜流动性增高及bcl_2和突变型p53基冈表达减少有关。 关键词益气养阴方 Experiment Experiment study on serum of the rats that Are Given ViQi Yangyin formula induces the Apoptosis of Human Leukemia cell lines K562 Speciality：Basic research ofintegrated TCM and western medicine Author：Zang Yunhua Tutor：Prof．1i Zhen Abstract Objective To explore the mechamism of treating leukemia with Yiqi Yangyin formula．Methods We hatched cell lines K562 with human leukemia by means of serological pharmacology．At the same time，Yiqi Yangyin formula is given to the rats． And then，we observe rats’serum’s effects on membrane fluidity，cell cycle and bcl-2 gene and mutant p53 gene expression of cell lines K562 by fluorophotometry，flow cytomeuy and immunohistochemic．Results Serum of the rats given Yiqi Yangyin formula at lower drug concentrations has little effect on the membrane fluidity．But at higher drug concentrations it can increase membrane fluidity of cell lines K562，this effect has the same tendency to serum of the rats given arsenical agents and that given HU．Serum ofthe rats given Yiqi Yangyin formula can increase membrane fluidity and S phase cells’percentage of cell lines K562 and decrease GO-G l phase cells’percentage， which is similar to the effect of the rats’serum that are given arsenical agents．Serum of the rats given Yiqi Yangyin formula down·regulates the expression of bcl-2 gene and mutant p53 gene，and in addition it induces the apoptosis of the cell lines K562． Conclusion With serum ofthe rats given Yiqi Yangyin formula，S phase cells of cell lines K562 increased，and then a