过量表达CDPK9对拟南芥耐逆性的影响-发育生物学专业论文.docxVIP

山东Iffi范大学碗士学位论文Abstract 山东Iffi范大学碗士学位论文 Abstract CDPK is a kind of Calcium-dependent protein kinases which iS first found in plants．It belongs to Ser／T11r protein kinases family，CDPK has a molecular weight of 40～90kDa，Mf+works aS its assist member。CDPKs arc defined by a carboxyl·-terminal calmodulin-·like domaim Mutational analysis indicates that the junction domain,which joins the kinase and calmodutin-like domains，contains。an autoinhibitor． 11le CDPK family represents diversity and choosable during its signal transduction gmeway．Reneently lots of inveStigatio璐into rice and arabidopsis r印rc∞m that the CDPK family is comprised of multiptegene family．Arabidopsis includes 12 subfamilies consisted of 34 members．As to rice，27 members． Furthermore most ofthem involve in adversity signal transduction．we have validated the expression of arabidopsis genes A￡CDPKl and AtCDPK2 a托toned up in drought and salt conditions(Urao ct a1．，1994)．In addition,Sheen(1996)make SUle that the arabidopsis genes AtCPKl0 and AtCPK30 involve in ABA and abiological si弘al transduetion． Thellungiella halophila is close relative to Arabidopsis which is also belong tO Crucifeme and has good genetic features such舔similar morphology,small genome size，short life cycle，hi曲seed number and an efficient transformation method． Howev骶,Thellungiella halophila is able to withstand dia／natic salinity shock叩to 500ram NaCI．础s plant does not produce salt酉ands or other complex morphological alterations either before or after salt adapmtiom Therefore，the study of function of thioredoxin．from Thellungiella halophila is quite significant for expatiating plant response mechanism of oxidative slress，understanding the metabolism alteration and adjustment of plant under stresses and expounding the stress tolerance metabolism． n壕CDPK9 gene Was obtained from ESTs(expressed sequence tags)acquired 丘om LZap-eDNA library of Thellungiella halophila in our lab．Through the expression in vitro and activity experiment we try to pr