回复吸水过程中发菜光合活性及相关转录本的变化-植物学专业论文.docxVIP

⑨ ⑨ MASTER STHE姗 在干燥脱水的过程中Rubisco和PSH反应中心DI蛋白受到损伤，在回复吸水过程 中这两种蛋白开始修复；psbAl和psbA2基因表达差异说明光合系统的重建是一个 多步骤的过程，尽管这种多步骤的意义和具体程序仍不清楚。干燥贮存长时间的发 菜在回复吸水24 h后，rbcL，r6cr，psbAl和psbA2转录丰度依然很低，这说明 长时间的贮存对DNA以及与转录相关的蛋白造成了比较严重的损伤，而这种损伤 可能与ROS的积累有关。 关键词：发菜；回复吸水；光合作用；光合电子传递；快速光响应曲线；Real-time RT-PCR：光合作用相关基因 Ⅱ AbstractNostoc Abstract Nostoc flagelliforme BometHah．is a terrestrial and highly desiccation-tolerant blue—green alga,being distributed in the arid or semiarid areas of dry desert and grassland． It has great economic value and is very significant for the ecology of the dry desert and grassland．Unfortunately its resources have been over．exploited due to the increasing market demands．As a result,the ecologicaJ balance of its habitats has been damaged， As a consequence，many scientists have paid great attention to its protection and a number of studies have been carried out since 1998．In this paper,we reviewed the studies ofⅣ-flagelliforme in recent years,and then investigated the physiological changes of AT．flagelliforrae having been driedly stored for different periods and the expression of its photosynthetic genes during rehydration． During rehydration,the weight ofN．flagdliforme increased in three phases谢th the times of 2 rain,0．5 h and 24 h．Owing to the physiological activities of living cells，the difference WaS observed in the third phase forN．flagelliforme dricd for 2 or 8 years． The photosynthetic activity was fully recovered within 24 h mhydration inⅣ． flagelliforme after 2 years of drying storage，but that of samples dried for 8 years WaS only 33．4％of the former samples after 24 h mhydratiom This indicated that longer storage in aryness required mole time for the photosynthetic recovery．The PSI activity WaS rocovered almost within 15 min．suggesting that their function was restored very quickly．However,the PSll activity was recovered mainly in the third phase．After 24 h rehydration,the chlorophyll fluorescence or PSH photosynthetic electron transport activity could not be d