急性白血病患者造血干细胞CD96分子表达的初步研究-内科血液学专业论文.docxVIP

华中科技大学硕士学位论文 华 中 科 技 大 学 硕 士 学 位 论 文 2 2 CD96 Expression on Hemopoietic Stem Cells in Acute Leukemia Abstract Objective This study was aimed to investigate the expression of CD96 leukemia stem cells in 91 patients with acute leukemia, and the results were analyzed with clinical pathological data. Methods Flow cytometry was used to detect CD96 molecule on the bone marrow mononuclear cell surface of 91 newly diagnosed patients with acute leukemia, and 15 healthy adults were served as normal controls. Results The average rate of CD96 expression was (53.27 ± 14.43) % in B-ALL stem cells + + + + + + (CD45 CD34 CD19 ); (87.54 ±10.02) % in T-ALL stem cells (CD45 CD34 CD7 ); and + - (49.88±5.68) % in AML stem cells (CD45+CD34 CD38 ); which was significant difference + compared with healthy controls (0.65 ± 0.59) %. After treatment, compared CD96 + group CR rate（52%）and CD96 group NR rate (10.6%）with healthy controls, which had significant difference. CD96 average rate in stem cells were detected after treatment. + Compared CD96 CR group （1.68±2.31）% with healthy controls, which had no difference. + However, compared CD96 NR group （62.39±26.29）% with healthy controls, which had 96significant difference (p＜0.05).The leukocyte, hemoglobin and platelet count in CD96+ group had no obvious difference than CD - ones. There was no change found in the field of molecular biology and cytogenetic between these two groups. 96 Conclusion CD96 expression is different in different type of leukemia. The positive expression of CD96 on bone marrow hematopoietic stem cells in patients with acute leukemia may be associated with primary drug resistance, replase and progression. The CD96 in hematopoietic stem cells of AL could be a helpful prognostic indicator in treatment response assessment. Key Words CD96; flow cytometry; Leukemia stem cell 独创性声明 本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成 果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或 集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文 中以明确方式标明。