急性肺栓塞大鼠肝细胞生长因子的变化-内科学专业论文.docxVIP

II II 组血浆 HGF 在造模后 1 h 开始升高，12 h 达到高峰，48 h 后下降至接近正常， 实验组与对照组比较，造模 1、6、12 和 24h 血浆 HGF 差异有统计学意义（P ＜0.05)；急性肺栓塞肺组织中 HGF 蛋白表达以阳性面积率表示，组织对照 组为（0.88±0.26）×10-2，组织实验组为（3.49±0.56）×10-2，两者差异有 统计学意义（P0.01）。 结论 急性肺栓塞时 HGF 明显升高，并于 12h 左右达到高峰；HGF 有望 作为一种新的生物学标记物用于急性肺栓塞早期诊断。 关键词 急性肺栓塞；大鼠；肝细胞生长因子；动物模型 III III CHANGES OF HEPATOCYTE GROWTH FACTOR IN RAT AFTER ACUT PULMONARY EMBLISM ABSTRACT Objective Deploy acute autoblood pulmonary embolism (APE) to manufacture rat pulmonary embolism animal model. Use ELISA to detect hepatocyte growth factor ( HGF) in blood. Use immunhistochemical method to observe the expression of Lung tissue HGF, and to observe the change trends of HGF. To Study the diagnosis value of HGF for pulmonary embolism and to investigate the mechanism of HGF increasing in acute pulmonary embolism. Methods 32 health adul SD rats（male and female） were randomly divided into 2 groups, plasma HGF detection group (16 rats) and organization HGF detection group (16 rats), each group were divided into controll group (8 rats) and experimental group (8 rats). Experimental group, model group, were deployed acute autoblood pulmonary thromboembolism aseptically to manufacture rat pulmonary embolism animal model. 1 mL blood were taken from the tail and into sterile catheter in sterile conditions, after 25 ~ 30 min in the room temperature and 10 min 70℃ water bath, a solid thrombus was formated. In the ultrasound-guided 4F sheath to expand the tube was inserted into the right PAGE PAGE VI atrium or right atrium near the inferior vena cava Department. Prefabricated embolus was sub-injected through the sheath to expand the tube until the rats occurred shallow and fast breath, irritability, wheezing, lips and ears cyanosis and other hypoxia symptoms, and the heart rate increased by about 30 times / min compared with preoperative, stop the injection and the modeling as prompted successful. Control group, sham-operated group, were injected the same amount of saline of autologous