急性肺炎模型中血浆氨基脲敏感型胺氧化酶活性的研究-生物化学与分子生物学专业论文.docxVIP

汕头大 汕头大学医学院硕士学位论文 汕头大 汕头大学医学院硕士学位论文 PAGE PAGE IV PAGE PAGE VII Abstract Semicarbazide-sensitive amine oxidases (SSAO) are a group of copper ，quinone and histidine residue containing amine oxidases which catalyze oxidative deamination reaction of amine compounds and are sensitive to semicarbazide. Its physiological and pathological function remains unclear. In human, SSAO is present either as soluble or as membrane forms located in circulation and many tissues and organs, especially in adipocytes, vascular endothelial and smooth muscle cells. It has been found that SSAO activity changes in some pathological conditions. For example, in the acute peritonitis, chronic dermatitis or pneumonia conditions, SSAO activity increased in diseased region. In addition, plasma SSAO activity increased in hepatocirrhosis patients. Interestingly, the protein expression and activity of SSAO were reported unchanged in acute lung inflammation, which are different from our expectations. In the present study, we chose suitable animal model for investigation of the variation of SSAO activity in the acute lung inflammation, and the regulation mechanism of SSAO. Main procedures and methods Established Wistar, SD rats, New Zealand rabbit model of acute pneumonia, selected and optimizated animal models for SSAO activity study . Evaluated the quality of the rabbit model of acute lung inflammation by animal symptoms observation, WBC and pathological section. After treated with different administration ways and doses of LPS, plasma SSAO activities were compared. Detected the variation of plasma SSAO activity after LPS treatment. Purified plasma for the endogenous substances. Results: The experiment proved that administration by ip or iv induced more side effects than intratracheal administration. Rats plasma SSAO activity is too low, so rabbit model was more ideal than rats. The direct administration by tracheal intubation is better than traditional tracheotomy intubation administration in