急性肾功能衰竭中Bcl-2促进肾小管上皮细胞早期修复的实验研究-外科学(泌尿外)专业论文.docx

蔓三呈堕奎堂竖主兰垡笙苎————英文缩写一览表 蔓三呈堕奎堂竖主兰垡笙苎———— 英文缩写一览表 英文缩写 英文全称 中文对照 A／rid Ampicillin 氨苄青霉素 ARF acute renal failure 急性肾功能衰竭 ATN acute tubular necrosis 急性肾小管坏死 Bcl．2 B cell lymphoma／leukemia一2 gene B 细胞淋巴淋巴瘤／白血病基因 CFU Colony forming unit 克隆形成单位 DAB Diaminotlezidine 二氨基联苯胺 DMSO dimethyl sulfoxide 二甲基亚砜 DEPC Diethyl pyrocarbonate 焦碳酸二已酯 FBS Fetal bovine serum 胎牛血清 FCM flow cytometer 流式细胞仪 G glycerol 甘油 G418 geneticin 遗传霉素418 H202 Hydrogen peroxide 过氧化氢 HK一2 human renal epithelial cells 人肾小管上皮细胞 mC Immunohistochemistry 免疫组织化学 IsH In situ hybridization 原位杂交 ITR long terninal repeat sequence 长末端重复序列 MCS multiclonal site 多克隆位点 MTT 3一(4，5一dimethyl一2·thiazoly)2，5一diphenyl一2H- 四甲基偶氮唑蓝 tetrazolium bromide OD optical density 光密度 PCNA proliferating cell nuclear antigan 增殖细胞核抗原 TUNEL Terminal Deoxynucleotidyl Transferase— 脱氧核糖核酸末端转移酶介 mediated Deoxyuridine Triphosphate Biotin 导的dUTP缺口末端标记 Nick End Labeling V virus 病毒 第三军医大学博士学位论文The 第三军医大学博士学位论文 The investigation on Bcl-2 in facilitating the recovering process of initial renal tubular epithelium injury in culture and acute renal failure Abstract BACKGROUND：The mortality in patients with acute renal failure has remained unchanged since the mid一1960s and remains high at approximately 50％．It is unlikely that the high mortality rate of the disease will be reduced until We have a better understanding of the cellular and molecular mechanisms of renal tubular cell injury and recovery．It has long been recognized that in acute renal failure，renal tubule cells die in a catastrophic breakdown of regulated cellular homeostasis．known as necrosis．Over the last decade there is an accumulating evidence suggesting the renal epithelial cell apoptosis contributes significantly to the pathogenesis of acute renal failure．A better understanding of the mechanisms of apoptosis could lead t0 safer and more specific therapeutic interventions for acute renal failure．In this study,we present evidence to support the hypothesis that apoptosis is an important patho