活血法、补益法对肝细胞增殖及周期的影响-中医基础理论专业论文.docxVIP

入肝而藏并司调节血量之功：肝血充足流通，才能柔软肝体，制约肝 入肝而藏并司调节血量之功：肝血充足流通，才能柔软肝体，制约肝 阳，维持疏泄功能的正常。 关键词：活血法，补益法；正常人肝细胞株L02；细胞增殖，细 胞周期 ABSTRACTPurpose：By ABSTRACT Purpose：By comparing the innuence of Da珊hen R(斌injection and Mincyetch Root injection on cell prol溉ra曲n锄d cell p鲥od of no衄al hIlm锄being’s livef cen strain U)2，we are imend t0 f盯mer lay the experirnental伊ound for the r锚eafch on the TCM th∞ry of liver storiIlg tlle blood and provide creative ideas柚d methods for纰ating liver diseases iII dinic． Method：By the adIninistration of directly adding Da璐h钮R00t injection and Mnkvetch Root injection 1respec帆ely with diabrent concentmtions imo cIllt哪e solution，we tal【e出e MTT method to t鹤t the di由￡c∞nt i11fluences of the two kinds of injectio璐on oen proli蛋eration of no姗al h衄蛆be她’s livef cell strain功2姐d the nowing type锄alysis ins嘶nt to ide州晦the di胁ent iⅡml蚰ces 0f the two kinds of injectio璐on ccH period of no珊al hu糊being’s liver cell stmin L02． R船ult：Fifsny，∞mpared to me blaIll【group，Da矗sh％R00t injecti仙 0bViously has positive e￡cect to the pmIifemtion of human being，s liver ccll s咖iIl L02(it ch蛳geS markcdly when the dmg concen仃ation in b100d plaslna is in 4，8，16，32 times，P0．05)．In addi廿on，w“h moI己using of tlle dosage，r也efe is a certain quaH锣一e脆ct relationship．More0Ver，in liver ceU strain L02，cells of G1 phase arc in dedine wllile cells of S粕d G2 ph弱es are in fise whi c：h compared to the raw铲oup，me柚s the cell period is nmch mofe in advance．Secondlycomp盯ed t0 blanl【gmup，Mill【陀tch R∞tⅫecti∞h勰no R∞tⅫecti∞h勰no evident e髓ct t0 the prol谁ra舶n 0f ceU stra血LD2， 唧t o舱of the dosage g吼驴that enrts positive rl巴∞如n．AlsO， 舡e曩把d by胁ctch Rc融埘em呱础s of G1 pha∞in nv盯∞n strain -eveal∞evident曲柚gewhile∞Us ofSpha∞areinfi∞柚d∞lls 0fG2 afc in d∞Ii鹋．T扯谴y’c0呷amd t0 n地group of MnJcve自ch R(Ⅺt Ⅻecti锄，幽e gmup of D姐sh∞R(埘t竭ection h勰皿Iuch more evident fIIn咖of pmmoting the ceⅡprolifera曲n and le耐ing oens to dr叫ate in advan∞． C0ndusion：①It is p0鹋ible nlat the method of a嘲a妇g b100d ci：响diI畦蚰to Iemove stasis is她alized th砷ngh enh蛐g也e ac曲