外源性硫化氢对百草枯致大鼠急性肺损伤细胞炎性因子的调节作用.docVIP

Exogenous hydrogen sulfide regulates the expression of inflammatory cytolines in the model of rat’s acute lung injury was caused by paraquat Abstract Purpose：By measuring the content of IL-10、IL-1β、ICAM-1 and NF-κB to observe the mechanism of H2S play a protection on lung through adjust inflammatory cytokines and hoping to provide the theoretical basis for clinical treatment of paraquat poisoning. Methods：Sixty healthy rats were randomly divided into 6 groups.The blank group：NaCl group （intraperitoneal injection of 0.9% NaCl）、The NaHS group (intraperitoneal injection of NaHS);Experimental groups :PQ group、PQ+low dose of NaHS（L group 0.78mg/kg）、PQ+middle dose of NaHS（M group 1.56mg/kg）、PQ+high dose of NaHS（H group 3.12mg/kg）.All rats were bleeded to death after PQ lavaged 24 hours；Observing the lung wet/dry weight，right lung tissue stained with HE，at high magnification observed the changes of pathological score of lung injury. Three experimental analysis methods ( Western Blot、ELISA and RT-PCR) were respectively used to measure the expression of NF-κB protein in the lung tissue、the content of IL-1βand IL-10 in the plasma of rats、the level of IL-1β、IL-10 and ICAM-1 mRNA in the lung tissue Results: General activity in rats、lung tissue pathology change and (IQA) score： At the optical microscope observed the HE staining of lung tissue section，between NaCl group and NaHS group did not see the pathological change,IQA had not obvious difference（P＞ ）.The lung tissue structure of PQ group was obviously damaged，there were a large number of alveolar interval fracture and mutual fusion , many red blood cells and inflammatory cells in the alveolar ,meanwhile alveolar septum visible inflammatory cell infiltration and septa in lung tissue changed into thickening,some of alveolar transparent membrane formation,IQA and（W/D）in PQ group was increased had statistically significantly (P＜0.01).In L、M and H groups most of structure of alveolar were intact and IQA、（W/D）was statistically si