白藜芦醇苷在大鼠体内的药代动力学研究.doc

第四军医大学硕士学位论文 to 0.25mL plasma. The samples were extracted twice with 2.0mL acetidin. The organic phase was transferred to a clean tube and evaporated to dryness at 50℃ under a gentle stream of nitrogen in the ventilation cabinet. The residue was dissolved in 50μL of methanol and 10μL was taken for LC/MS/MS analysis. (2) mL glucuronidase solution(2000 U/mL)was added to 0.25mL plasma and incubated at 37℃ for 24h in water bath shake. The sample was treated detected according to step (1). 3. The model of intestinal tract perfusion in situ was set up. The intestina parva of 10cm in length from the point 5cm below the duodenum was perfused by intubation, and then the perfusate samples were collected at different points(10, 20, 30, 40, 50, 60min) via portal vein. The concentration of resveratrol and polydatin were detected by the method of LC/MS/MS. At the same time, the sample was treated with glucuronidase to detect the concentration of glucuronidated resveratrol and polydatin. 4. The model of liver perfusion in situ was set up.The drug was poured into portal vein, and then the perfusate samples were collected at different points(10, 20, 30, 40, 50, 60min) via inferior vena cave. The concentration of resveratrol and polydatin were detected by the method of LC/MS/MS. At the same time, the sample was treated with glucuronidase to detect the concentration of glucuronidated resveratrol and polydatin. RESULTS: 1.Take the ratio of peak area of polydatin and resveratrol to peak area of internal standard genistein separately for Y-axis, and the concentration of polydatin and resveratrol for X-axis to built the working curve .The typical regression equation to assay the concentration of the polydatin in the rat plasma was Y = 0.0543X-0.032，γ=0.9999，n=6. The linear range was 0.4-200ng/mL and the fixed lower quantity was 0.4ng/mL. The typical regression equation to assay 6 第四军医大学硕士学位论文 the concentration of the resveratrol in the rat plasma was Y =0.0366X+0.1138，γ =0.9999，n=6. The l