狼蛛食性分析及控制害虫效能的评价-生态学专业毕业论文.docxVIP

ABSTRACTUsing ABSTRACT Using the dominant species Pardosa pseudoannulata as the predator and the Nilaparavate lugens，Sitobion avenae，and Nephotettix bipunctatus with high incidence and large number of farmland harm as target pests，the best and most optimal combination of environmental factors for the successful predation rate of spider was explored in both natural and artificial ecosystems，two experimental paddy fields in Changsha and Dongkou，Hunan Province，China as natual ecosystem，and three levels orthogonal experiment as man-made ecosystem，using the target pest’S nucleic acid residues extracted from the residue of spider’S digestive tract as template，the speciese and number of target pest were detected and analyed by real-time quantitative PCR．The following results were obtained： 1．In the paddy field as natural ecosystem，Pardosa pseudoannulata is the dominant species with hunting widely，preying lots of only living pets，and staying long in the field．There is a strong correlation between the spider and its target pest planthoppers regarding the seasonal ecological distribution(rChan舀ha=O．6407，r D。ngk。u∞unty=0．62720)． 2,The preying process of Pardosa pseudoannulata was affected by the light color，light intensity，and temperature in the microenvironment in the paddy field．Orthogonal test showed that the most significant impact of predation efficiency of the Pardosa pseudoannulata is III 万方数据 temperature，followed temperature，followed by light color and light intensity．Further statistical analysis by the three factors and three levels orthogonal test showed that the best condition for Pardosa pseudoannulata’preying was that temperature is 26℃，light intensity 1 5Lx，and ambient color is green． 3．A method for identifying prey species was Established following qualitative PCR using the DNA fragments remaining in spider’S digestive tract．Based on the method，the DNA fragment of a single species of preying target in spider Pardosa pseudoannulata’S digestive residue Can b