芦竹的组织培养研究-植物学专业论文.docx

Study Study on the tissue culture of Arundo donax Master Candidate：Chen Cen Grade：20 1 3 Supervisor：Li Bo—lin Major：Botany Research Direction：Plant Physiology Abstract Based on Arundo donax were collected from field growing and tissue culture plantlets of laboratory condition as the explants．Plant tissue culture technique was applied to study the effects of explant type and different exogenous hormone treatment on the rapid propagation system of A．donax．This research Can provide theoretical basis on the establishment and perfection of the rapid propagation system，breeding and industrialization ofhi曲quality cuttings ofA．donax．The main test results were as follows： 1．The test results of selection of explants ofA．donax： (1)The best explant and the size of field plantlets were the 0．5 cm unilaminar young leaves． (2)The best explant and the size of tissue culture plantlets were the 1 cm tender stem segments upon the tillering node of 20d aseptic seedling． 2．11le test results of callus induction ofA．donax： (1)m best leaf callus-inducing medium of field plantlets was MS+I．5 mg／L 2，4一D+0．5 m叽NAA+0．05 mg／L KT，and the callus rate was92．22％． (2)The best tender stem segment callus—inducing medium of tissue culture plantlets was MS+2．5 mgrL 2,4-D+I．0 mg／U NAA+0．05 mg／U KT，and the callus rate was 93．33％． (3)Both young leaves of field plantlets and tender stem segments of tissue culture plantlets，dark condition was suitable for their callus induction． 3．圈b test results of callus proliferation ofA．donax： (1)Thebestleafcallusproliferationmedium offieldplantletswasMS+I．0mg／L 2,4-D+o．1 medL NAA+0．05 mg／L KT．and the multiplication coefficient was2．76． (2)The best tender stem segment callus proliferation Medium of tissue culture plantlets was MS+0．5 mg／L 2,4-D+0．5 mg／L NAA+0．05 mgm KT，and the multiplication coefficient was 1．76． 4．The test results of callus differentiation ofA．donax： (1)ne best leaf callus differentiation medium of field plantlets was MS+0．2 mg／L NAA+2．0 mg／L 6一BA，and