山牡荆药材质量控制研究-药物分析学专业论文.docx

PAGE PAGE 10 并为山牡荆药材质量标准的建立提供参考。 关键词： 山牡荆；化学成分；TLC；HPLC Study on quality control of the herbs of Vitex quinata （Lour.）Will. ABSTRACT Objective: Guangxi Vitex quinata （Lour.）Will. medicinal materials by different regions as the research object，establish the quality evaluate method for Vitex quinata （Lour.）Will. The paper will provide the reasonable foundation for scientific appraisal and effective control the quality of Vitex quinata（Lour.）Will. Methods: The chemical constituents of medicinal powder pretest； Microscopic identification of original plants were collected；By orthogonal experiment to optimize the extraction process of Vitex quinata （Lour.）Will.； TLC was used for the identification of medicinal materials of Vitex quinata （Lour.）Will.；In accordance with the provisions of Pharmacopoeia appendix method for regular inspection and extract of Vitex quinata（Lour.）Will. determination；Determination of the content of effective components of ecdysterone in medicinal materials of Vitex quinata（Lour.）Will. by HPLC method. Results: Chemical composition test results: May contain carbohydrate, flavonoids, saponins, steroids, grease and other components of Vitex quinata （Lour.）Will. in; Microscopic identification: Vitex quinata（Lour.）Will. powder starch grains, visible threaded conduit, bordered pit vessel, stone cell, cork cell and fiber, Transverse section of root powder in the visible duct, The transverse section of the stem cells were scattered in the visible stone；TLC identification method：Thin layer chromatography of Vitex quinata（Lour.） Will.，Ecdysterone clear spots，In contrast medicine chromatogram and standard chromatogram corresponding position show the same black spots. The best extraction method with 50% methanol ultrasonic extraction No. four sieve powder 60min.Determination of the content of effective components of Ecdysterone Vitex quinata（Lour.）Will. herbs in the HPLC method: Detector for ultraviolet detector, The detection wavelength was 247nm,