山茶油掺伪检验方法研究-农产品加工及贮藏工程专业论文.docxVIP

II II 2、测定了 15 个批次山茶油的主要脂肪酸 GC 指纹图谱，建立了纯山茶 油的主要脂肪酸对照 GC 指纹图谱，找到 6 个共有峰。采用夹角余弦法计算 其相似度，15 个批次山茶油与其对照图谱的相似度≥0.9989。测定了纯山 茶油与不同掺入比例的芝麻油，葵花油，花生油，玉米油和菜籽油的二元 混合油的主要脂肪酸 GC 指纹图谱，通过计算掺伪油脂和山茶油对照 GC 指 纹图谱的相似度，对掺伪油脂进行检验。该方法对山茶油中掺芝麻油、葵 花油和玉米油的检出限均为 10%；对山茶油中掺花生油和菜籽油的检出限 分别为 20%和 30%。 3、 采用 DSC 对山茶油及其掺伪样品进行扫描，寻求规律，找到快速 鉴别山茶油真伪的方法。研究了山茶油及山茶油分别掺入芝麻油、葵花油、 花生油、玉米油和菜籽油二元混合油的 DSC 曲线特征，山茶油熔融吸热峰 出现在-6.65±0.54℃，其结晶放热峰在-36.54±0.55℃，其他植物油及其山茶 油掺伪油样的吸热和放热峰温度均低于纯山茶油。由单因素显著性分析结 果知（p0.05），当芝麻油、花生油和玉米油的掺入量达 5%，当葵花油和 菜籽油的掺入量达 10%时，其主要吸热峰和放热峰的起始温度（Ton）、峰 值（Tpeak）和终止温度（Toff）均与纯山茶油的有显著差异，其主要的熔融 吸热峰和结晶放热峰显著变宽并显著往低温度处移动，表明 DSC 可以作为 有效鉴别山茶油真伪的方法，该方法具有良好的精密度（RSD＜7%）和线 性范围（R2=0.9993）。采用逐步多元线性回归法以冷却图谱的热力学参数 为变量建立山茶油定量鉴别模型，得到 5 个相应的定量鉴别模型，对模型 进行可行性检验，其平均误差为±1.2620%，可以满足检测要求。 关键词：山茶油 鉴伪 指纹图谱 差示扫描量热法 多元逐步回归 III III STUDY ON THE METHODS FOR DETERMINATION OF ADULTERATED CAMELLIA OIL ABSTRACT The camellia oil is a kind of healthy edible oil with high nutritional value. Lack of olive oil in China, but rich in camellia oil. Now the camellia oil is becoming more and more popular all over the world, and the acreage, yield and demand of camellia oil are growing. Because the price of the camellia oil is so high, Some peddlers made an illegal means, mixing cheap oils into camellia oil to seek high profits, which greatly damaged the interests of consumers. Guangxi is one of the province is rich in camellia oil, in view of the adulteration phenomenon in camellia oil, 3 characteristics such as acid value, peroxide value, iodine value were analyzed, using 15 batches of Semi refined camellia oil as tested samples. Five common vegetable Oils, including sesame oil, sunflower oil, peanut oil,corn oil,and rapeseed oil were mixed into camellia oil at different ratios(5%,10%,20%,30%,40%), respectively. In order to establish camellia oil adulteration identification method, GC fingerprint and differential scanning calorimetry (DSC) were applied to identify camellia oil adulteration.Quantitative identification model of identificati