施氏假单胞菌N2对含氧多环芳烃的生物降解特性研究-环境科学专业论文.docxVIP

西安建筑科技大学硕士论文 西安建筑科技大学硕士论文 Study on Characteristics of biological degradation for the Oxygen-containing Polycyclic Aromatic Hydrocarbons by Pseudomonas Stutzeri N2 Speciality: Environmental Science Author : WangFei Advisor: Professor Nie Maiqian Abstract The processes which the microorganism would degradate PHAs will generate stable oxygen-containing intermediates in the natural environment. Most of their biological toxicities are stronger than maternal ring, and easily to accumulate. They have a lot of harm to human health through the ecological food chain.1-hydroxy-2-naphthoic acid is more typical compounds. So the behavior which the compounds could be mineralized rapidly and thoroughly plays a practical significance role in restoring for environmental pollution by oxygen-containing Polycyclic aromatic hydrocarbons. Firstly, the bacteria has been screened on metabolizing 1- hydroxy -2- naphthoic acid in this thesis. Secondly, the condations of improving degradating rate are studied for 1-hydroxy-2-naphthoic acid.We identified the intermediating products of 1-hydroxy- 2-naphthoic acid by Pseudomonas Stutzeri N2 using GC-MS and speculated that the d egradation mechanism based on the identification results lastly. The results are as follo wing. Pseudomonas Stutzeri N2 can grow on 1-hydroxy-2-naphthoic acid as the sole carbon source and energy. The bacteria may degradate1-hydroxy-2-naphthoic acid rapidly when the pH is in 7.5 and the concentrations of Fe2+,Ca2+and Mg2+ are 0.05 mmol/L, 0.1 mmol/L and 0.1mmol/L in medium. Acetone, glycerol, glucose, octane, n-octane etc which are co-metabolism carbon source in the process of 1-hydroxy-2-naphthoic acid and Vitamin B1 as auxin by N2 bacteria. It can promote the mineralization of the toxic substances. The degradation rate is increased by 5.76%-48.8% in the media adding co-metabolism carbon sources and vitamin B1. But also they can make the transformation products of the maximum a