施氏假单胞菌N2降解酚类污染物的特性及机理研究-环境工程专业论文.docxVIP

西安建筑科技大学硕士学位论文 西安建筑科技大学硕士学位论文 西安建筑科技大学硕士学位论文 西安建筑科技大学硕士学位论文 Characteristics and Mechanism of Pseudomonas stutzeri N2 degradation of phenolic pollutants Speciality: Environmental engineering Author: ZhaoJing Advisor: Professor Nie Maiqian Abstract Because of its source variety in environment, the phenolic compound is a kind of widely existing environmental pollutant. It is toxic, thus, it brings great harm to the environment. The treatment of microorganisms is an important way to clean up this pollutant from the environment. It has been reported many strains can degrade phenol, however, the phenol degradation, particularly its ring-opening cracking, is rather difficult. In line with the preliminary study, our group had isolated and screened a strain Pseudomonas stutzeri N2. This research optimizes conditions, under which bacteria N2 can degrade phenol by the ring-opening fragmentation pathway, so as to promote the decomposition and complete mineralization of toxic phenolic compounds. Based on the previous studies, this thesis probes the degrading potential of Pseudomonas stutzeri N2 to phenol pollutants under conditions of using phenol as the sole carbon source, phenol and cometabolism as the carbon source, and nitrophenol as the carbon source, etc. The results are as follows: When the Pseudomonas stutzeri N2 grew on phenol as a sole carbon source, the Pseudomonas stutzeri N2 could bear 1000 mg/L phenol. When the initial concentrations of phenol were within 50mg/L~800mg/L, there was no toxicity to the Pseudomonas stutzeri N2 which growth rapidly. And the net Growth △ OD550nm of 40h can reach 1.27. The synchronous degradation rate of phenol was 100%. But its main degradation pathways with phenol as a sole carbon of the Pseudomonas stutzeri N2 were the hydroxylation and methylation pathways, which were not beneficial for phenol mineralization and would generate the typical carboxylated product: p-Hydroxybenzoate and the subsequent methylated products. And this failed t