纳秒脉冲电场消融乳腺癌的实验研究.docVIP

纳秒脉冲电场消融乳腺癌的实验研究 ［ ］目的探索纳秒脉冲对乳腺癌高转移细胞株MDA-MB-231的治 疗效果及其分子机制。方法纳秒脉冲电消融乳腺癌细胞及荷瘤小鼠后， 检测接受纳秒脉冲治疗（实验组）及未接受纳秒脉冲治疗（对照组）的小 鼠肿瘤细胞增殖、周期和凋亡相关指标，观察肿瘤的体积、生长形态及速 度等的变化。结果纳秒脉冲在体外可以显著降低细胞活性，诱导细胞凋 亡，表现为实验组CCK-8细胞活性抑制率与对照组比较，差异冇高度统计 学意义（P〈 0.01）,且各实验组间差异均有高度统计学意义（P 0.01）； 投射电镜和线粒体电势实验观察到细胞出现凋亡，抑制肿瘤生长。结论 纳秒脉冲可以通过诱导乳腺癌细胞MDA-MB-231的凋亡而对肿瘤进行消融。 ［关键词］乳腺癌；MDA-MB-231；纳秒脉冲;消融 ［中图分类号］R730. 5 ［文献标识码］A ［文章编号］1673-7210 （2015） 06 （a） -0004-06 Experiment study of nanosecond pulsed electric fields ablate breast cancer XIE Guilinl CHEN Luyan2 CHEN Xinhua2 WEN Hao3 CHEN Xinmei4 Department of General Surgery , the Second Hospital of Shaoxing , Zhejiang Province , Shaoxing 312000 , China ； Department of General Surgery, the First Affiliated Hospital of Zhejiang University, Zhejiang Province, Hangzhou 310000, China； Department of General Surgery, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China； 4. School of Pharmacy, Shandong University of Traditional Chinese Medicine, Shandong Province, Ji nan 250355, China [Abstract] Objective To investigate the treatment effect of nanosecond pulsed electric fields (nsPEFs) on high 1 y-metastatic human breast camcer cell lino MDA-MB-231 in vitro and in vivo the and the possible molecular mechanism. Methods After treatment of nsPEFs in different parameters, the apoptosis, proliferation and invasion of MDA-MB-231 cells in rats of nanosecond pulse therapy (experimental group) and non—nonosecond pulse therapy (control group ) were examined with CCK-8 , TUNEL , f1ow cytometer , transmission electron microscopy (TEM) . The volum of tumor size, groth pattern and speed were observed? Results In vitro, nsPEFs inhibited the cell viability and incluced cell apoptosis. The cell viability of CCK-8 in the experimental group was reduced significantly compared with control group, the difference was statistically significant (P 0.01)， and the differences among different experimental group were statistically signif