赶黄草提取物对大鼠胆汁淤行积性肝纤维化tgf-βsmad途径的影响.docxVIP

西南医科大学硕士学位论文Influence 西南医科大学硕士学位论文 Influence of The Extracts from Penthorum chinese Pursh On TGF一13／Smad Signaling Pathways in Cholestasis Liver Fibrosis Rats Abstract：Objectives To investigate the influence of the extracts from Penthorum chinese Pursh on pathological changes and therapeutic benefits in cholestasis liver fibrosis rats through TGF—p／Smad signal pathway．Methods： Cholestasis liver fibrosis model was set upby common bile duct ligation of both ends and then a middle cut—off in male SD rats．Total 40 male SD rats with successful modeling were randomly divided into four groups：common bile duct ligation group， high and low dose(49／kg‘1一d 1，29／kg-1·d‘1)of the extracts from Penthorum chinese Pursh groupgroup，ursodeoxycholic acid group(30mg／kg)with 1 0 rats in each group．Another 1 0 male SD rats were served as control group randomly．Medicines were all taken by gavage， normal and common bile duct ligation group rats were given equal dose of normal saline．111e rats were anaesthetized at the end of 2nd and 4th week after operation to take the blood from heart for detecting not only the serum level of(hyaluronic acid，HA)，(1aminin，LN)，(collagen type IV，CIV)，but also liver function indexes(ALT、AST、ALB、DBIL、 TBIL)，meanwhile，partial hepatic tissues of all 5 groups rats were taken for biopsy．If the nude mice died in the experimental process，An autopsy should be done in time．All the hepatic tissues were fixed in 1 0％neutral 3 万方数据 西南医科大学硕士学位论文formalin，embedded 西南医科大学硕士学位论文 formalin，embedded in paraffin and stained with HE to observe the pathological changes of hepatic tissue and the degree of hepatic fibrosis，besides the dyeing parts and staining intensity of TGF一13 1 (transforming growth factor 9 1，TGF-p 1)，Smad3，Smad7 and伐-SMA were determined by immunohistochemistry Envision method．Results： @Serum Level of HA，LN，pclII，clVamong ursodeoxycholic acid group and both high and low dose of the extracts from Penthorum chinese Pursh group were all inferior