小鼠卵子突变Om基因及其修饰基因的相关研究.docxVIP

文章编号：1009 ? 0568（2008）03 ? 0001 - 07 小鼠卵子突变（O加丿基因及其修饰基因的相关研究 赵卫东 (河南农业大学牧医工程学院，河南郑州 450002 ) 摘要 近交系小鼠DDK具有一种被称为DDK综合症的奇异特性:如同品系内交配一样，DDK雄鼠能够使其它近交系雌鼠 正常受孕。但是，当DDK雌鼠与其它近交系雄鼠交配时，F1受精卵在妊娠3~5日因胚泡形成障碍而死亡，使得DDK雌鼠几 乎不孕。这种特性是由于DDK卵子细胞质物质和其它品系精子因子之间存在的一种不亲和性引起的，它们二者均受到位于 小鼠第11条染色体的卵子突变(Ovton Mutant或O加丿位点上的一对等位基因所控制本文介绍的新研究成果包括.?第一发 现了存在于C51BL/6(B6)品系小鼠遗传背景中的新的修饰基因.其使得杂合型丿雌鼠的胚胎死亡率增加.并提出了解 释修饰基因作用机理的等位基因排斥表达假说;第二论证了 DDK品系本身遗传奇景中不具有使杂合型 5 / +)雌鼠胚胎 死亡率降低的修饰基因,?第三证明了小鼠遗传背景中，不存在通过杂合型(5 / +)公鼠的精子影响KO” /6J雌鼠胚胎 死亡率高低的修饰基因,?第四通过不同亚种间近交系小鼠的杂交，得到了如下重大发现??在欧洲起源的DDK品系(Musm 中图分类号：Q953 文献标识码:A Studies on the 0 vnm M utant (Om) and ItsM odffier Genes h the M ouse Zhao Weidong (College of Aninal Husbandry and Veterinaiy Science, Henan Agricultural University, Zhengzhou, Henan 450002) Abstract hibred strain of m ice DDK shows a unique pioperty knovn as t)DK syndicme? DDK males can noirnally fertilize the fe2 males of other inbred strains as in intra strain mating, while the females ofDDK strain are mostly infertile when mated with males of odi2 er inbred strains, because of the deatli of Fl zygotes due to failure in blastocyst fonnatbn Tliis is caused by an incanpatibility between the cytoplasnic substance ofDDK eggs and the speni factor of other strains, and botli of than are con tio lied by the alleles at the oviin mutant (Om) bcus on mouse Chinnosonie I L Tlie present paper reviews the fbllowing new research results: 1) there exists a new modifier gene which increases tlic an bionic mortality in the hctciozygous (Om / +) females having C57BL /6 (B6) background genes in large pioportions, and a hypothesis of the allelic exclusion was proposed for explaining the participatbn of a modifier gene; 2) the re exists no a mod ifie r gene wh ich dec rea se s the em b ryon ic mo rtility in the he te iozygDU s (Om / + ) fem ales having DD K backgiounc genes in lai^e pioporlions; 3) there exists no modifier gene which influences the embiyonic mor(alily of DDK (Om/Om) female、 through the jpem of the hetc