10 pabp表型 PABP亚细胞定位 4bGUS RNA-seq分析.ppt

Work report col atprmt4a4b atpab2 atpab4 atpab8 atpab24 atpab48 atpab28-79 atpab28-139 4a4batpab24 4a4batpab48 4a4batpab28 Total leaf number of the atprmt4a4b and atpab mutants under long day condition Corresponding FLC expression level will be detected. col atprmt4a4b atpab24 atpab48 atpab28-79 atpab28-139 col atprmt4a4b atpab24 atpab48 atpab28-79 atpab28-139 Vernalization － Vernalization ＋ Total leaf number of the atprmt4a4b and atpab mutants after the treatment of vernalization Corresponding FLC expression level will be detected. PRMT4-dependent arginine methylation do not affect the subcelluar distribution of PABP1 (Brookk et al.,2011) XF1551/XF1552/XF1553 transformed atprmt4a4b 5 lines of each type of transformation were observed. Confocal images will be taken. XF1551: pCAMBIA1300-AtPABP2 promoter: AtPABP2 genomic DNA-6xGly-GFP-HA- AtPABP2 terminator XF1552: pCAMBIA1300-AtPABP4 promoter: AtPABP4 genomic DNA-6xGly-GFP-HA- AtPABP4 terminator XF1553: pCAMBIA1300-AtPABP8 promoter: AtPABP8 genomic DNA-6xGly-GFP-HA- AtPABP8 terminator The subcellular localization of PAB2/4/8 are not changed under atprmt4a4b background XF1556 transformed atprmt4a4b 10 lines of seedlings were observed. 2 lines of inflorescence is under detecting XF1556: pCAMBIA1300-AtPRMT4b promoter: AtPRMT4b genomic DNA-GUS-HA-TAG-AtPRMT4b terminator Gus staining result of AtPRMT4b For seedlings, the SAM, young leaves and roots are significantly stained. Better picture will be taken using the T3 generation seedlings cultivated on the MS plates. Gus staining result of AtPRMT4b Anther and young seeds Gus staining result of AtPRMT4b 2 3 4 5 6 7 8 9 Young seeds Summary of High-throughput RNA Sequencing Analysis 　 　 　 　 　 　 Library Total reads Total match Unique match Multiple match Reads mapped to genome Reads mapped to splicing junctions Col20117760(80.3%) 96.9%) 631263(3.1%)74.3%) 5169245(25.7%) atprmt4a4b20527315(79.5%)96.6%) 707777(3.4%) 1533152