红锥不同种源叶片表型性状变异分析.doc

17个红锥种源叶片性状变异分析 张凤良1,2 张方秋2﹡ 潘 文 2 徐 斌2 段安安1 王有兵1,2 朱报著2 （1.西南林业大学 昆明 650224；2.广东省林业科学研究院） 摘 要 对中国红锥主要分布区17个种源的叶片形态性状进行测定，分析了不同产地红锥叶片的遗传变异，结果表明，种源间15个叶片性状的变异系数为0.64～26.09%，且在种源内或种源间差异均达到显著水平，表明不同种源间和种源内叶片性状的遗传变异较丰富；种源间表型分化系数的变幅为8.96%-37.65%，均值为22.97%，小于种源内变异（77.03%）；叶片长、叶片宽、宽基距、脉左宽及叶面积等性状与经度和年均温呈极显著负相关，与海拔高度呈极显著正相关。研究结果为红锥良种分类、选育和快速繁殖育苗提供了科学依据。 关键词 红锥 种源 叶片性状 遗传变异 Variation Analysis on Leaf Traits of 17 Castanopsis hystix Provenances Zhang Fengliang1,2 Zhang Fangqiu2＊ Pan Wen2 Xu Bin2 Duan Anan1 Wang Youbing1,2 Zhu Baozhu2 Southwestern Forest University, kunming,650224；2. Guangdong Academy of Forestry) Abstract Leaf traits of 17 provenance resources in the major distribution region of the whole country were determined, and the genetic variation of C. hystix leaves from different producing areas was analyzed.The results showed that variance coefficient of 15 leaf traits ranged from 0.64～26.09%, and significant difference of leaf traits existed among provenances of C. hystix ,which indicated that variation of leaf traits in C. hystix provenances were abundant.The range of phenotypic differentiation coefficients among provenances were from 8.96% to 37.65% and the average value was 22.97%,which were lower than those within the provenances(77.03%).The correlation analysis of each leaf character in C. hystix Provenances suggested that leaf length, leaf width, left leaf width and leaf erea showed extremely significant positive correlation with altitude ,while those showed extremely significant negative correlation with longitude and average annual temperature. The study had provided a certain basis for the classification,breeding and Rapid propagation of nursery of C. hystix. Key words Castanopsis hystix, Provenance, leaf trait, genetic variation 植物群体在长期适应环境的过程中产生各种形式和程度的形态变异，植物个体的形态差异是由本身的遗传背景和所处环境共同作用的结果，也是植物适应其生存环境的表现形式[1]。形态特征变化常常作为遗传变异的表征[2]，如何根据表现型差异来反映基因型差异，是应用形态学标记检测遗传多样性的关键。叶片的表型变异亦是植物遗传变异和环境差异的共同反 基金项目：国家林业科研公益专项基金“红锥育种体系营建技术研究”（200904013）