组织工程软骨细胞体外培养技术要点.docVIP

Deparlnient of Orthopaedies, Nanjing General Hospital, Nanjing Military Area Command of Chin esc PI-A, Second Military Medical University of Chinese PLA, Nanjing 210002, Jiangsu Province, China Deparlnient of Orthopaedies, Nanjing General Hospital, Nanjing Military Area Command of Chin esc PI-A, Second Military Medical University of Chinese PLA, Nanjing 210002, Jiangsu Province, ChinaZhang Yong*, Studying for doctorale. Alic nding physician, DcpartnKnl of Orthopaedics, Nanjing General Hospilah Nanjing Military Area Command of Chinese PL A. Second Military Medical University of Chinese PI-A, Nanjing 210002, Jiangsu Province, China yjzy22hllc@yalux). 组织工程软骨细胞体外培养技术要点承 张勇，赵建宁 In vitro techniques of chondrocyte culture in tissue engineering Zhang Yong, Zhao Jiarnning Abstract： It has been a novel clinical way to repair damaged articular cartilage with autologous chondrocytes cultured in vitro using cartilage tissue engineering technique. There have been many articles about the techniques of chondrocytes culture in recent years? After summarizing and analyzing the involved articles, factors on chondrocytes cultured in vitro were reviewed, including dimension, stress, cell density and oxygen tension, etc. Chondrocytes cultured in ihwdimensional with multi-factors in vitro can promote quality and quantity of cartilage in tissue engineering? But factors on chondrocytes culture are so complicated that further researches will be needed? Zhang Y、Zhao JN. In vitro techniques of chondrocyte culture in tissue engineering.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008; 12(42):8340-8343(China) [ HYPERLINK /zglckf/ejoumal/upfiles/08-42/42k-8343(ps).pdf/zglckf/ejoumal/upfiles/08-42/42k-8343(ps).pdf] 摘要：通过组织工程技术，采用n身软骨细胞体外増殖构建软骨來修复关节软骨损伤，在临床上已成 为一种有效的新方法。近年來,有关体外软骨细胞培养技术的研究较多。通过对入选文献进行分析、 整理，对够响体外构建软骨的因素包括培养空间、应力、细胞密度和氧张力等方而进行分析，体外采 用三维多条件联介培养软骨细胞可以改善构建软骨的牛物品质。但是影响软骨细胞牛长的因素众多复 杂,在体外如何更好地构建软骨,有待进一步研究。 关键词：组织工程：软骨细胞：细胞培养 张勇，赵建宁.组织工程软骨细胞体外培养技术要点[J]?中国组织工程研究与临床康复，2008, 12(42):