灵芝多糖和多肽对亚硝化反应抑制作用的研究.docVIP

PAGE PAGE 29 灵芝多糖和多肽对亚硝化反应抑制作用的研究 摘要：本实验分别用超声法和超声协同酶解法提取灵芝中的多糖和多肽，研究不同浓度的灵芝多糖提取液和灵芝多肽提取液对亚硝化反应的抑制作用，并比较在等量条件下，不同提取方法得到的灵芝多糖和多肽的阻断和清除能力。结果表明：1.灵芝多糖对亚硝化反应有一定抑制作用，但并不显著，超声法提取的多糖最大阻断率为10.54%，最大清除率为11.50%，超声协同酶解法提取的最大阻断率为32.86%，最大清除率为32.53%，等量条件下，超声协同木瓜蛋白酶水解得到的多糖对阻断、清除效果均最好；2.灵芝多肽对亚硝化反应具有明显的抑制作用，超声法提取的多肽最大阻断率为71.44%，最大清除率为56.00%，超声协同酶解法提取的多肽最大阻断率为98.14%，最大清除率为84.86%，等量条件下，超声协同纤维素酶水解得到的多肽阻断效果最好，超声协同木瓜蛋白酶水解得到的多肽清除效果最好。 关键词：灵芝；多糖；多肽；阻断；清除 Study on the inhibitory effects of Ganoderma lucidum polysaccharides and peptides on nitrosation reaction Abstract: This experiments were used by ultrasonic method and ultrasonic assisted enzymatic method extraction of polysaccharide and peptides in Ganoderma Iucidum，to study the ability of the disconnecting nitrosamine synthesis and scavenging sodium nitrite with different concentrations of Ganoderma lucidum polysaccharides and peptides. And compared under the same conditions，obtained by different extraction methods of polysaccharides and peptides from Ganoderma Iucidum. The results demonstrated that the Ganoderma lucidum polysaccharides had certain inhibition on nitrosation reaction，but the effects were not significant. The capability of disconnecting nitrosamine synthesis with nitrosamine synthesis was 10.54%，and the capability of scavenging sodium nitrite was 11.50%. The capability of disconnecting nitrosamine synthesis with ultrasonic assisted enzymatic method was 32.86%，and the capability of scavenging sodium nitrite was 32.53%. Under the equal conditions，ultrasonic assisted papain hydrolysis of polysaccharides on the nitrosation reaction had the best effect. On the other hand，the results demonstrated that the Ganoderma lucidum peptides had certain inhibition on nitrosation reaction. The capability of disconnecting nitrosamine synthesis with nitrosamine synthesis was 71.44%，and the capability of scavenging sodium nitrite was 56.00%. The capability of disconnecting nitrosamine synthesis with ultrasonic assisted enzymatic method wa