阿霉素对K562细胞Gfi1表达的影响及其与相关凋亡基因关系的研究.docVIP

阿霉素对K562细胞Gfi1表达的影响及其与相关凋亡基因关系的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：阿霉素对K562细胞Gfi1表达的影响及其与相关凋亡基因关系的研究 1 文2：Y盒结合蛋白1对白血病K562细胞多药耐药基因1表达的调控 9 1 材料与方法 10 2 结 果 13 3 讨 论 15 参考文摘引言： 16 原创性声明（模板） 17 文章致谢（模板） 17 正文 阿霉素对K562细胞Gfi1表达的影响及其与相关凋亡基因关系的研究 文1：阿霉素对K562细胞Gfi1表达的影响及其与相关凋亡基因关系的研究 Effect of Adriamycin on Gfi1 Expression in K562 Cells and Its Relatiohip with the Relevant Apoptotic Genes Abstract The study was purposed to explore the effect of adriamycin (ADM) on K562 cells in vitro and the mechanism of expression changes of relevant apoptotic genes and oncogene Gfi1.　The apoptosis was assayed by flow cytometry (FCM) and the DNA electrophoresis; the expression changes of Gfi1、Bcl2、bax mRNA and protein were detected by RTPCR and FCM after K562 cells were treated with different concentratio of ADM for 24 hou. The results showed that when K562 cells were treated with mg/L ADM for 24 hou, the typical apoptotic DNA electrophoresis band of K562 cells were observed with the dose increasing. When concentration of ADM was and mg/L, the expression of Gfi1 decreased and the expression of bax increased; when concentration of ADM was mg/L, the expression of Bcl2 was not found to be significantly changed, the levels of Bcl2 mRNA and protein were of no statistical difference. When dose of ADM was higher than mg/L, the percentage of apoptotic K562 cells decreased with cell necrosis. It is concluded that at certain range of concentration, apoptosis or necrosis of K562 cells can be induced by ADM, the percentage of apoptosis, the changes of expression of Bcl2, bax and Gfi1 depend on the dose of ADM. The mechanism of apoptosis in K562 cells induced by ADM may be related to suppression of Gfi1 oncogene and activation of expression of bax gene. Key words ADM; K562; apoptosis; Gfi1; Bcl2; bax J Exp Hematol 2007; 15(2):278-282 阿霉素（adriamycin, ADM）在细胞凋亡中起着重要的作用,国内外有不少学者利用阿霉素成功地诱导了细胞凋亡，但对其作用机制还不完全清楚。为此,我们利用阿霉素诱导急性髓系白血病细胞株K562细胞凋亡,旨在了解阿霉素对