STR－PCR分析嵌合体在同种异基因造血干细胞移植中的应用.docVIP

STR－PCR分析嵌合体在同种异基因造血干细胞移植中的应用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：STR－PCR分析嵌合体在同种异基因造血干细胞移植中的应用 1 文2：接骨合剂在同种异体骨修复骨缺损过程中BMP2基因的表达 9 1 材料与方法 9 2 结果 11 3 讨 论 12 参考文摘引言： 12 原创性声明（模板） 14 文章致谢（模板） 14 正文 STR－PCR分析嵌合体在同种异基因造血干细胞移植中的应用 文1：STR－PCR分析嵌合体在同种异基因造血干细胞移植中的应用 Application of Chimerism Analysis to Allogeneic Hematopoietic Stem Cell Traplantation by STRPCR Abstract The aim of this study was to analyze chimerism, evaluate the status of engraftment and predict the outcome of allogeneic hematopoietic stem cell traplantation (alloHSCT) by multiple short tandem repeat (STR) amplification using fluorescence labeling polymerase chain reaction (PCR) combined with capillary electrophoresis. Peripheral blood and bone marrow in 27 patients who received myeloablative allogenetic cell traplantation were collected before and after traplantation in different times. 10 and 7 different STR marke were coamplified in a single reaction by using a commercial AmpFTR Profiler Plus/Cofiler plus PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI prism 310 Genetic Analyzer with capillary electrophoresis. The Genescan and Genotype soft ware were used for size calling and quantification of peak areas. The formula to calculate donor chimerism values was based on the different allelic distribution type between donor and recipient. The results showed that donor chimerism was similar by the two methods. The median number of informative alleles was (4-9) by Profiler Plus and (2-6) by Cofiler Plus. The donor alleles appeared in 26 patients on day 28 post traplantation. One patient was not observed to appear donor alleles. 14 patients with 100% donor chimerism (DC) had stable engraftment and they still survive in free leukemia. 9 patients had utable mixed chimerism (DC: 0%-%), and 5 of them relapsed after alloHSCT, 6 patients died. Decrease of donor chimerism appeared prior to graft rejection and disease relapse. T