尾加压素Ⅱ对体外培养大鼠肾成纤维细胞的促增殖作用临床医学.docVIP

尾加压素Ⅱ对体外培养大鼠肾成纤维细胞的促增殖作用临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：尾加压素Ⅱ对体外培养大鼠肾成纤维细胞的促增殖作用临床医学 1 1 材料与方法 2 2 结果 4 3 讨论 5 文2：人参皂苷Rg1对体外培养胚胎神经干细胞增殖作用 6 1 材料与方法 7 2 结果 9 3 讨论 10 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 尾加压素Ⅱ对体外培养大鼠肾成纤维细胞的促增殖作用临床医学 文1：尾加压素Ⅱ对体外培养大鼠肾成纤维细胞的促增殖作用临床医学 【Abstract】Objective To observe the effects of uroteinⅡ(UⅡ) on the proliferation of the renal intetitial fibroblasts(RFB) of rats and explore the role of UⅡ in the renal intetitial fibrosis. Methods RFB from Wistar rats were isolated and cultured. Cell number was observed by MTT assay. Cell cycle was evaluated by flow cytometry (FCM).Results With the increase of urotein Ⅱ concentration, the optical deity at 490 nm of RFB as shown by MTT assay was fit increased but then decreased, and remained at a significantly higher level in the cells treated with 1×10-9 or 1×10-8 mol/L UⅡ compared with those in control group(P＜). With the treatment of 1×10-10，1×10-9，1×10-8，1×10-7 mol/L UⅡ, the S phase cell percentage were significantly higher, and the G0G1 phase cell percentage was significantly lower than those in control group(P＜，P＜), but the indices in 10-7 mol/L UⅡ group were not significantly different from those of control (P＞).Conclusio UⅡ can directly induce RFB proliferation and affect the cell cycle, which suggests the crucial role of UⅡ in the renal intetitial fibrosis. 【Key words】Urotein Ⅱ; Renal intetitial fibroblasts; Proliferation 尾加压素Ⅱ(Urotein Ⅱ，UⅡ)是一种生长抑素样环肽，最早从硬骨鱼脊髓尾部下垂体中分离而来，缩血管效应是内皮素1(ET1)的10倍多〔1〕。近年国内外的研究相继发现UⅡ并非仅有血流动力学作用，其在肾脏的高表达有可能有重要的意义〔2〕。目前UⅡ对肾间质纤维化的作用研究尚未见报道。本研究观察了UⅡ对大鼠肾成纤维细胞增殖、细胞周期的影响，旨在探讨UⅡ在肾间质纤维化中可能发生的作用。 1 材料与方法 主要试剂 体重100 g Wistar大鼠由吉林大学实验动物中心提供，DMEM培养基(Gibco)，标准胎牛血清(天津灏洋生物技术有限公司)，Urotein(UⅡ)(Sigma)，免疫组织化学SABC试剂盒和DAB显色剂(武汉博士德公司) 大鼠肾成纤维细胞的培养与鉴定 大鼠肾间质成纤维细胞从正常Wistar大鼠肾髓质中培养获得。 参考 Rodemann等〔3〕的方法进行分离培养。具体步骤为：将肾髓质切成1 mm3小块，加入%胰酶37℃消化10～15 min，将消化后组织悬液过200目筛网，滤液于1 000 min离心10 min，弃上清，用培养液洗涤两次，加入含20%胎牛血清的培养液，置37℃、5%CO2培养箱中培养。根据长出细胞形态学和免疫学表型确定为成纤维细