罗格列酮对糖尿病大鼠肾脏IL-6、IL-8、TNF-α表达的影响.docVIP

第39卷第6期第802页 华中科技大学学报(医学版) Vol.39 No.6 P. 802 2010年12月 Acta Med Univ Sci Technol Huazhong Dec. 2010 罗格列酮对糖尿病大鼠肾脏 IL-6、IL-8、 TNF- α 表达的影响* 王玉梅1, 黎慧清2△, 李贞琼1, 罗长青, 刘建社1 华中科技大学同济医学院附属协和医院 肾内科，2内分泌科，武汉 430022 摘要：目的 观察罗格列酮对糖尿病大鼠肾脏炎症相关因子 IL-6、IL-8 和 TNF-α 表达的影响。方法 雄性 SD 大鼠 被分为正常组、正常+罗格列酮组、糖尿病组、糖尿病+罗格列酮组。所有大鼠均检测生化指标、尿白蛋白排泄率，及肾 组织Ⅱ-6、IL-8、TNF- α mRNA 和蛋白水平。结果 罗格列酮干预的糖尿病大鼠和未干预组相比，肾组织 IL-6、IL-8、 TNF- α表达显著降低，尿白蛋白排泄减少，但血糖、肌酐无明显变化。结论 罗格列酮可能通过抑制炎症相关因子的表 达，从而降低尿白蛋白的排泄以保护肾脏，并且其作用独立于降糖之外。 关键词：糖尿病肾病； 炎症因子； 罗格列酮 中图分类号：R587.1 DOI:10.3870/j.issn.1672-0741.2010.06.016 Effects of Rosiglitazone on IL-6,IL-8 and TNF-α Expression in the Kidney of Diabetic Rats Wang Yumei,Li Huiqing2,Li Zhenqiong et al Department of Nephrology,2Department of Endocrinology,Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022,China Abstract Objective To observe the effects of Rosiglitazone on inflammatory cytokines expression in the kidney of diabetic rats. Methods Forty male SD rats were randomly divided into four groups:normal control group,normal control+Rosiglitazone group,diabetes group,diabetes+Rosiglitazone group. Diabetic nephropathy in rats was induced by intraperitoneal injection of streptozotocin(STZ,70 mg/kg)and the rats were killed after treatment for 8 weeks. Biochemical markers and urinary albumin excretion were measured. The expression levels of IL-6,IL-8 and TNF-a mRNA and protein were detected by real-time PCR and Western blot,respectively. Results As compared with untreated diabetic rats, Rosiglitazone significantly reduced the IL-6,IL-8 and TNF-a expression levels,and urinary albumin excretion in the kidney of diabetic rats. Meanwhile, there was no significant change in blood glucose and creatinine. Conclusion Rosiglitazone might improve renal function partly through inhibiting t