酶联免疫法分析糖尿病患者血清人巨细胞病毒抗体水平.docVIP

·1838 · · 论 著 · 酶联免疫法分析糖尿病患者血清人巨细胞病毒抗体水平 林伟良，刘文虎，徐冉行，凌志强 【摘要】 目的 探讨人巨细胞病毒 (HCMV) 感染与糖尿病的关系。方法 利用酶联免疫法(捕获酶联法和间 接酶联法)检测727例糖尿病(85例1型糖尿病，642例2型糖尿病)患者及230例健康体检者(正常对照组)血清 HCMV-IgM、IgG、IgA 水平，并比较不同性别、年龄、病程的糖尿病患者HCMV-IgM、IgA、IgG 的阳性率。结果 1 型糖尿病患者 HCMV-IgM、IgA 及 IgG 阳性率〔分别为10.59%、11.76%、92.94%〕及2型糖尿病患者 HCMV - IgM、IgA 及 IgG 阳性率〔分别为11.21%、11.68%、92.83%〕与正常对照组〔分别为0.87%、1.30%、77.39%〕比 较，差异均有统计学意义 (P0.05)。 年龄30岁及病程1年的糖尿病患者，血清HCMV-IgG 阳性率与其他年龄 组、病程组的患者比较，差异均有统计学意义 (P0.05)。 结论 HCMV 感染在糖尿病进展中起着重要作用，年龄小 或病程短的糖尿病患者既往感染者少。 【关键词】 糖尿病；酶联免疫吸附测定；巨细胞病毒 【中图分类号】 R587.1 【文献标识码】 A 【文章编号】1007-9572(2009)10-1838-03 En zyme -linked Immunosorbent Assay in Analysis of Human Cytomegalovirus Antibody in Diabetic Patients LIN Wei - liang,LIU Wen-hu, XU Ran-xing, et al. Minglou Community Health Services Centers,Ningbo 315040,China 【Abstract 】 Objective To explore the relationship between HCMV infection and diabetes. Methods Levels of HC- MV-IgM,IgG,IgA were determined by enzyme -linked immunosorbent assay in 727 diabetic patients (85 with type I,642 with type Ⅱ)and 230 healthy subjects(normal control group). Results The positive rates of HCNV-IgM,IgA, IgG were 10.59%,11.76%,92.94%,respectively, in type I group, and 11.21%,11.68%,92.83%,respecitively, in type Ⅱ group, and 0.87%,1.30%,77.39%,respecitively, in control,the difference was significant(P0.05). Patients less than 30 years old or with below - 1- year course were significant different from other age groups or patients with other disease courses in HCMV-IgG positive rate(P0.05). Conclusion Primary HCMV infection rates are significantly higher in youn- ger patients and/or patients having recently suffered from diabetes mellitus. HCMV infection is important in the pathogenesis of diabetes mellitus and its mechanism should be further studied. 【Key word 】 Diabetes mellitus; Enzyme -linked immunosorbent assay; Cytomegalovirus 糖尿病 (diabetes mellit