脐血干细胞移植在糖尿病足治疗中的应用：附二例报告.docVIP

·2551 · · 专 题 研 究 · 脐血干细胞移植在糖尿病足治疗中的应用：附二例报告 杨华强，李东升，杜玲，袁亚红，姜铧 【摘要】 目 的 观察脐血间充质干细胞移植治疗糖尿病足的疗效和安全性。方法 将脐血间充质干细胞多点注 射到自愿接受干细胞移植的2例糖尿病足患者的病变下肢，细胞数(3～7)×10?/L, 每点0.3～0.5 ml, 每点间距3 cm×3 cm, 肌肉组织丰富的部位可分层注射。移植术后第1天～3个月定期观察患者的临床症状及各项指标的变化并 进行综合分析。 结果 脐血间充质干细胞移植后3个月进行评价，2例患者下肢疼痛均明显缓解、皮温升高、皮肤凉 感消失、间歇性跛行明显改善、足部溃疡愈合。踝肱指数、经皮氧分压较治疗前有明显升高， CT 血管造影显示治疗后 病变下肢均有丰富的侧支血管生成。移植后2例患者均未出现严重并发症和明显不良反应，无一例患者行截肢术。结 论 脐血间充质干细胞移植治疗糖尿病下肢缺血性血管病是一种安全、有效的手段，可使部分患者免除截肢。 【关键词】 糖尿病足；脐血干细胞移植；治疗结果 【中图分类号】 R587.29 【文献标识码】 D 【文章编号】1007-9572(2010)08-2551-03 Application of Cord Blood Stem Cell Transplantation in Diabetic Foot YANG Hua -qiang,LI Dong-sheng,DU Ling, et al. Center of Cell Therapy, Taihe Haspital,Yunyang Medical College,Shiyan 442000,China 【Abstract] Objective To observe the clinical effect of umbilical cord blood mesenchymal stem cell(UCB-MSC) transplantation on diabetic foot(DF). Methods UCB- MSC suspension [cell numbers(3～7)×10?/L,0.3～0.5 ml per point] was injected into affected lower extremity through multipoint muscles with a distance of 3 cm ×3 em between each point. Clinical symptoms and indexes were observed from day 1 to months 3 after operation. Results Three months after UCB-MSC transplantation, lower extremity pain relieved, skin temperature increased, intermittent claudication ameliorated, ulcer healed, ankle-brachial index(ABI)and transcutaneous partial pressure of oxygen increased. Angiography showed plenty of collateral vessel generatings. Both patients had no severe complications and clear side - effects. No amputation of limbs was performed. Conclusion UCB- MSC transplantation is an effective and safe method in treatment of diabetic ischemic angiopathy of lower limbs, which may exempt limb amputation for some patients. 【Key words] Diabetic foot; Cord blood stem cell transplantation; Treatment outcome 作者单位：442000湖北省十堰市，郧阳医学院附属太和医院细胞治疗中心(杨华强，李东升，杜玲，袁亚红);郧阳医学院附属 太和医院血液科(姜铧) 通讯作者：杨华强，442000湖北省十堰市，郧阳医学院附属太和医院细胞治疗中心； E-mail:yanghuag