水稻抽穗期QTL DTH2的图位克隆和功能分析.docVIP

  水稻抽穗期 QTL DTH2 的图位克隆和功能 分析# 吴玮勋1，江玲1，钟铮铮2，陆广文1，万建民1** 5 10 15 20 25 30 （1. 南京农业大学作物遗传与种质创新国家重点实验室，南京 210095； 2. 中国农业科学院作物科学研究所，北京 100081） 摘要：开花时间（作物中指抽穗期）是决定生长季节和植物适应特定自然环境的地区适应性 的重要生态性状。水稻是起源于热带地区的短日作物。本文鉴定了一个在长日照条件下促进 抽穗的微效数量性状位点 DTH2，并对其进行了分子克隆。互补和 RNA 干扰实验证实 DTH2 编码一个 CONSTANS-like 蛋白。DTH2 蛋白定位在细胞核内并具有转录自激活活性，并且 DTH2 的表达受节律钟调控。我们发现通过诱导成花素基因 Heading Date 3a (Hd3a) 和 RICE FLOWERING LOCUS T 1 (RFT1) 来促进抽穗，并且独立于已知的开花集成子 Heading date 1 (Hd1) 和 Early heading date 1 (Ehd1)，体现了微效 QTLs 在作物育种中起重要作用。 关键词：水稻；DTH2 克隆；抽穗期；功能分析 中图分类号：S511.S330.2+5 Map-based cloning and function analysis of QTL DTH2 for heading date in rice (Oryza sativa L.) WU Weixun1, JIANG ling1, ZHONG Zhengzheng2, LU Guangwen1, WAN Jianmin1 (1. State Lab of Crop Genetics and Germplasm Ehancement, Nanjing Agricultural University, NanJing 210095; 2. Cro Science Institute, CAAS, Beijing 100081) Abstract: Flowering time (heading date in crops) is an important ecological trait that determines growing seasons and regional adaptability of plants to specific natural environments. Rice (Oryza sativa L.) is a short-day plant that originated in the tropics. We report here the molecular cloning and characterization of DTH2, a minor-effect quantitative trait locus (QTL) that promotes heading under long-day conditions (LDs). Complementary test and RNA interference assay confirmed that DTH2 encodes a CONSTANS (CO)-like protein. DTH2 protein is targeted to the nucleus and has transcriptional activation activity and DTH2 expression is regulated by the circadian clock. We show that DTH2 promotes heading by inducing the florigen genes Heading Date 3a (Hd3a) and RICE FLOWERING LOCUS T 1 (RFT1), and it acts independently of the known floral integrators Heading date 1 (Hd1) and Early heading date 1 (Ehd1), demonstrating an important role of minor-effect QTLs in crop adaptation and breeding. Keywords: Rice; DTH2 cloning; Heading date; Function analysis 35 0 引言 成功的现代农业依赖于作物更好地适应于当地的环境和栽培范围的扩张，主要取决于合 适的开花时间（在水稻中开花时间指抽穗）[1]。