藏灵菇增殖条件的优化.pdfVIP

l 机d价总 Scienιe and Technology 0/ Food Industry 生物主意 藏灵菇增殖条件的优化 赵小元，文鹏程，艾对元，杨敏，梁琪，米兰，张炎，张卫兵导 (甘肃农业大学食品科学与工程学院，甘肃兰州 730070) 摘 要:采用羊因素实验、部分因素实验和中心组合设计对藏灵菇在复合培养基中的增殖条件进行优化。结果表明， 培养温度和初始接种量对藏灵菇增殖的影响显著，得到的最优条件为·接种量I.I9毛、温度23.2C、初始pH6.48 、培养时 间 22h 时下，增殖率的预测极大值为263.04%。验证实验表明，在该条件下藏灵菇增殖率为252.679毛:t4.54%。 关键词:藏灵菇，增殖，优化 Optimization of the proliferative condition of Tibetan mushroom ZHAO Xiao-yuan ,WEN Peng-cheng ,AI Dui-yuan ,YANG Min ,LIANG Qi ,MI Lan ,ZHANG Yan , ZHANG 明lei-bing* CCollege of Food Science and Engineering , Gansu Agricultural University , Lanzhou 730070 , China) Abstract: Using single factor experime时，fractional factorial experiment and central composite design to optimize the proliferative condition of Tibetan mushroom in compound media ,the result showed that temperature and inoculation amount had significant effects on the proliferation of Tibetan mushroom. The 0 optimal condition as follows: when inoculation amount of 1.1 % (w/v) ,temperature of 23.2 C , initial pH6.48 and replacing time of medium 22h ,the prediction of the maximum value was 263.04%. The result of verification test showed that the proliferative rate of Tibetan mushroom would be 252.67% :t4.54% under the condition Key words: Tibetan mushroom; proliferation; optimization 中图分类号:TS201 文献标识码:A 文章编号:1002-0306(2012)20-0238-04 藏灵菇是 种白色或浅黄色的胶质状物质，外 大规模工业化生产，而通过在培养基中原加营养物质， 形无规则，多为米粒、花椰菜和薄皮状。藏灵菇常常 可以促进藏灵菇的快速增殖，同时培养基的更换时 被误认为是源自亚洲的红茶菌和开菲尔粒，但它们 间、培养温度、菇体的冲洗条件以及基本营养物浓度