潜阳育阴颗粒含药血清对AngII诱导HUVEC损伤NO的影响及其机制研究.docVIP

  潜阳育阴颗粒含药血清对 AngII 诱导 HUVEC 损伤 NO 的影响及其机制研究# 王欣彤1，方祝元2，严士海2** 5 10 15 20 25 30 35 40 （1. 南京中医药大学第一临床医学院,南京,210029； 2. 南京中医药大学附属医院,南京,210029） 摘要：目的 探讨潜阳育阴颗粒含药血清对 AngII 诱导 HUVEC 损伤 NO 的影响及其机制。 方法 建立 AngII 诱导的人脐静脉内皮细胞凋亡损伤模型，采用 TBA 法检测 NO 含量，Western Blot 法检测 eNOS 及 iNOS 蛋白表达，ELISA 法检测 NADPH 氧化酶水平，用 SPSS17.0 软 件对数据进行统计学分析。结果 模型组 NO 及 eNOS 蛋白表达较正常组降低，NADPH 氧 化酶则明显升高；缬沙坦组及潜阳育阴颗粒高剂量组 NO 明显升高，而潜阳育阴颗粒低剂量 组则无显著性差异；予缬沙坦组及潜阳育阴颗粒干预后，eNOS 蛋白表达显著升高，NADPH 氧化酶含量明显下降；各组 iNOS 表达差异不明显。结论 潜阳育阴含药血清对 AngII 诱导 体外培养的 HUVEC 损伤具有保护作用。 关键词：潜阳育阴颗粒；血管紧张素 II；一氧化氮 中图分类号：R285.5 Study of Impact Mechanism that QianYangYuYin Granules Containing Serum on AngII Inducing HUVEC Injuring NO Wang Xintong1, Fang Zhuyuan2, Yan Shihai2 (1. Refers to Postgraduates of The first clinical medical college of Nanjing University of Traditional Chinese Medicine,,Nanjing,210029; 2. Jiangsu Province Hospital of TCM,Nanjing,210029) Abstract: To study the impact mechanism that QianYangYuYin(QYYY) granules containing serum on AngII inducing HUVEC to injure NO. Mythology Establish the model of AngII-induced apoptosis in human umbilical vein endothelial cells, Use the measure of Western Blot to defect the content of eNOS iNOS. ELISA to detect the content of NADPH oxidase. Perform Statistical analysis by using of the software SPSS17.0. Result The protein expression of NO and eNOS in the model group were decreased compared with the normal group while NADPH oxidase increased in an obvious growth.The NO in the valsartan group QYYY with high doses increased obviously but in the lower doses group, there is not obvious difference. After valsartan group QYYY granules has its effect, protein expression of eNOS increased obviously and the content level of NADPH oxidase decreased quite much; While expression of iNOS were not seen differently.Conclusion QYYY-containing serum on AngII induced injuring cultured HUVEC out of body has protective fuctions. Key words: QianYangYuYin Granules ; AngII；NO 0 引言 高血压作为一种常见病，高发病，可引起心、肾等重