两性生殖卤虫16S rDNA分子系统学研究.pdfVIP

AgriculturalScience＆Technology，2011，12(5)：659-662 Copyright⑥2011，InformationInstituteofHAAS．Allrightsreserved AgriculturalBiotechnology MolecularPhylogenyofBisexualArtemiaBasedon 16S rDNA 、 YIN Hong ，GUANNi，FUYu·ting CollegeofLifeSciences，HebeiUniversity，Baoding071002 Abstract [Objective]Theresearchaimedatinvestigatingthetaxonomicpositionandphylogeneticrelationshipofbisexualbrineshrimps． JMethod116SrDNAofthreespeciesofbisexualANemiafromChinawasdetermined；thehomologoussequencesbetweenthemand11relative speciesofArtemiafrom GenBankwerecompared；themolecularphylogenetictteewasconstructedbyMegaMicrosoffusingArtemiopsisstefans- sonfasoutgroup．1ResultlArtemiapersimiliswastheprimalgroupingenusArtemia；Artemiafranc~canaandANemiamonicaweretheevolved groups；Artemiaurmiana，ArtemiasinicaandotherArtema『speciesfr0mChinasharedaclosegeneticrelationship． 『Conclusion]Basedonthe 16SrDNA sequenceofthem，thephylogeneticrelationshipsofthesebisexuaIArtemiaspecieswereA．persimilis--~A．urmiana．A．sniicaand A．tibetiana-~A．tunisiana--,A，monica_+A ．Franciscan． Keywords BisexuaIArtemia：16SrDNA：Molecularphylogeny ANemia (brineshrimp)belongstoArthropoda，Crustacea． onANemiamolecularsystematicsbyusing 16SrDNA asa ge- Branchiopoda，Anostraca，Artemiidae．ANemiaisahighsalt-re- neticmarkerhasbeenfound．Thereby，16SrDNA wasusde in sistantsmallcrustaceanIivingintheinlandsaltIakesandcoast- presentstudyasgeneticmarkertoinvestigatethetaxonomicpo- aIsaltpondsallovertheworldatawiderangeoftempera- sitionandphylogeneticrelationshipof bisexualANemiaspecies． ture．Theeggs．naupliiaandadultofbrineshrimpsarerichin protein．whichare thepretty ideaInaturaIfoodoftheyoung MaterialsandMethods fish。shrimp．crab and caneffectively improve suwivaIrates Materials 0fthem．The existing ANemia species is mainly identified TheArtemiaeggswereh