绿玉树茎段组织培养再生体系的建立（摘要）.pdfVIP

AgriculturalScience＆Technology，2011，12(3)：379—381，465 Copyright⑥2011，InformationInstituteofHAAS．Allrightsreserved AgriculturalBiotechnology EstablishmentofTissueCultureRegenerationSystems LIU Zhao-liang，HE Jue-min ，CHEN Biao，LIANG Jia-xian AgriculturalBiotechnologyInstitute，GuangdongOceanUniversity，Zhanjiang524088 Abstract 『Objective]TheaimwastostudytheconditionsoftissuecultureregenerationseedlingbyusingthestemsegmentsofEuphorbetiru- caIllanddeterminetheoptimum cultureconditionofeachculturestage．soastoprovidereferencesforthefactoryproductionandrelativestudy oftissuecultureseedlingofE tirucaIl1．M『ethod]TakingthestemsegmentsofE tirucaIllasexplants，theeffectsofvariousmediumsongermi- nationrate．multiplicationcoefficientandrootingratewerestudied． 『Result]Theoptimum Inductionmediumofgerminationculturewas1／2MS+ NAA 0．02mg／L+6一BA 1．0mg／L．withdifferentiationrateof89．7％：thebestsubculturemedium was1／2MS+NAA0．02mg／L+6-BA 0．60 mq／L+AD3．Omg／L，withmultiplicationcoefficientof5．70；theoptimum rootingculturemediumwas1／2MS+NAA0．40mg／L+IBA0．4mg／L， withrootingrateof100％ andtransplantingsurvivaIrateof80％． 『Conclusion]ThetissuecultureconditionsofstemsegmentsofE tirucaIllwere determinedprimarily． 、 Keywords Euphorbiatirucalli：Stem segment；Tissueculture Belonging to Euphorbia，Euphorbiaceae，Dicotyledone- ProvincewasastestmateriaIandwasprovidedbyAgriculturaI ae．Angiospermae．Euphorbia tirucalliis also named milk Bi0technOIOqV Institute，Guangdong Ocean University after bush．rubbereuphorbia and soon… ．Originating inAfrica。 germplasm resourcesselection． E tirucalliwasintroducedinAsia．Europe．AmericaandAus- Disinfectionmethodofexplants traliaandmainly．distributesin Guangdong。Hainan，Yunnan ORe-yearstem segments(3—4cm 0fE tirucaIllwere andotherprovincesinChina【．Withrichwhiteoryellow