茉莉酸甲酯诱导的小麦白粉病抗性与9个抗病相关基因表达间的关系.pdfVIP

AgriculturalScience＆Technology，2011，12(4)：504—508 Copyright⑥2011，InformationInstituteofHAAS．Allrightsresewed AgriculturalBiotechnology The Relationship of MethylJasmonate Enhanced Powdery Mildew Resistance in W heat and the Expressionsof9DiseaseResistanceRelatedGenes NIU Ji．shan’ LIU Jing ，MA Wen-bin。 U Qiao-yun’，WANG Zheng—yang’，HEDe-xian’ ， ， 1．NationalCentreofEngineeringandTechnologicalResearchforWheat，HenanAgriculturalUniversity ／KeyLaboratory ofPhysiological EcologyandGeneticImprovementofFoodCropsinHenanProvince，Zhengzhou450002；2．CollegeofLifeScience，HenanAgriculturalUni— versity，Zhengzhou450002 Abstract f0biective]Thisstudywascarriedouttodeterminethejnductioneffectofiasmonicacid(JA)Oilpowdery mildew resistancejn wheat．theactivationeffectontheexpressionsofplantdiseaseresistancerelaledgenes，andtoinvestigatetherelationshipbetweentheinduced resistanceandthegeneexpressionpatterns． M『ethod1Threepowdery mildew susceptiblecultivarsof”ChineseSpring”，”Pumai9”and “ Zhoumai18“typicallyrepresentingdifferentphenotypesinthefieldwereemployed．Thepowdery．mildewwasassessedbydetachedleafassay． andrealtimequantitativeRT-PCRwasusedtodeterminetheexpressionpa~ernsof9diseaseresistancereIatedgenesofPR1(PR1．1)，PR2 (I3，1—3glucanase)，尸月3 (chitinase)，PR4 (wheatwin1)，PFI5 (thaumatin-likeprotein)，PR9(TaPER0，peroxidase)，PR10， GL a (germin．1ike)andTa．JA2(iasmonate—inducedprotein)inleafofthethreecultivars．1ResllltIMeJAapplicationenhancedthepowderymildewre— sistancesof”ChineseSpring”．”Pumai9”and”Zhoumai18”．Theinducedpowderymildewresistancecouldbedetecledfrom 12hc096hafter MeJA treatment．andthepeakvaluewasat24h．Thoughthereweredifferencesbetweenthethreecultivars，MeJAsignificantlyeffectontheex· pressionsofthe8 diseaseresistancereIa{edgenesexcept7_aGL尸2a andthepeakvalueswereat12h．24h or48h aftertreatmerits．The strongestactivationofMeJA wason PB9 and PR1thattheire