宁夏六盘山区黄牛杂交改良群体GHR基因多态性分析（摘要）.pdfVIP

AgriculturalScience＆Technology，2011，12(3)：413—416 Copyright⑥2011，InformationInstituteofHAAS．Allrightsreserved AgriculturalBiotechnology Analysis on Polymorphisms ofGHR Genee i。nn I。m一一 proved Hybrid Yellow Cattle Groups frOm Liupan MountainAreainNingxia ZHANG Chun-zhen’ LIJu-cai ，YANG Yi’ YU Jian-yong。 ， ， 1．SchoolofAgriculture，NingxiaUniversity，Yinchuan750021；2．NingxiaAcademyofAgricultureandForestrySciences，Yinchuan750002； 3．OfficeofAgro·farmingandScienceandTechnologyofJingyuanCounty，Jingyuan756400 Abstract 『0biective]Thepaperaimedtoanalyzethepolymorphism ofthegrowthhormonereceptor(GHR)inimprovedhybridyellow cattle groupfr0mLiupanMountainareainNingxiaAutonomousRegion．soastoprovidetechnologicalbasisforhybridimprovement．1MethodlPolymer- asechainreaction—restrictionfragmentlengthpolymorphisms(PCR—RFLP)technologYwascarriedouttoexaminepolymorphismsofGHRgeneof70 individuals． R『es山t]Thetargetfragmentof338bDwasamplified．ThePCRproductdigestedbyrestrictionenzvmeAluIshowedpolymorphisms． Thefrequenciesofthetwogenotypes(AA ．BB)were75．71％ (53individuals)and24．29％ (17individuals)，respectively． ConclusionI 岫geno- typesofGH开 geneweredetectedinimprovedhybridyellow cafflegroupsfr0m LiupanMountainareajnNingxia． Keywords Hybridyellow cattiegroup：Growthhormonereceptorgene；Polymorphism HalaIbeefandmuttonisoneofthefivestrategicIeading traitsofanimals．Therefore，ithasgreatsignificancetostudy industries of agriculturaleconomy in Ningxia． Liupanshan thegeneticvariability ofGHR geneand ＼tsrelationshipwith Mountainareaisacattlebreedingbasewithalonghistory．In economictraitsofanimals_d-4j． recentyears．withthecontinuouspromotionofecologicaIcon- Inpresentexperiment．polymerasechainreaction—restric- structionprojectofreturningfarmlandtograss，thegrassand tionfragmentlengthpolymorphism (PCR—RFLP)analysiswas Iivestockind