南极适冷菌Rheinheimera sp.97产抗菌活性物质发酵条件的优化.pdfVIP

AgriculturalScience＆Technology，2011，12(5)：776—780 Copyright⑥2011，InformationInstituteofHAAS．Allrightsreserved PlantProtection Optimization ofFermentation Medium and Condi-- tionsofAntibioticActive Substances Produced by ■I ■ ■ ■- ■ ■ ■ ■ Antarctic PSyChrOtrophiC aCtenUm ne，n力e，m e，-a sp．97 LIHe’一 ． LINXue-zheng ．LIUTong-jun 1．KeyLabofMarineBioactiveSubstances，SOA，FirstInstituteofOceanography，SOA，Qingdao266061；2．FoodandBiologicalEngineer- ingDepartmentofShandong InstituteofLightIndustry，Jinan250353 Abstract 10hjectiveJTheaimwastooptimizethefermentationmediumandconditionsofantibioticactivesubstancesproducedbyAntarctic psychrotrophicbacterium Rhemheimerasp．97．1Method1Single-factorexperimentando~hogonaltestwereadoptedtooptimizethefermenta． tionmedium ofantibioticactivesubstancesproducedbyAntarcticpsychrotrophicbacterium 开．sD．97．whilethefermentationconditionswereop． timizedbysingle-factorexperiment．{ResultITheoptimumfermentationmediumfortheantibioticactivesubstancesproductionwasasfollows： tryptone3．0g／L，amm0nium sulfate1．0g／L，starch2．0g／L，NaCI15．0g／L Theoptimizedfermentationconditionswereasfollows：thestart． ingpH ofmediumwas8 o，fermentationtemperaturewas10oC， liquidvolumeinErienmeyerflaskwas30％ (V／ andinoculationamountwas 1％ (V／ ．Undertheoptimizedfermentationmediumandconditions，theantibacteriaJactivityofR sp．97was；ncreasedby18．1％ fConclu． sionlThisstudyhadprovidedbasisfortheantibioticactivesubstancesproducedbyAntarcticpsychrotrophicbacteriumR．sp．97． Keywords Antarcticbacteria；AntibacteriaIactivity；Fermentationconditions：Optimization Polarregions hasuniquegeographicaIandclimaticfea— riaIactivityto humanopportunistic pathogen such asEsche— tures．and jtiscovered by snow alIyearround exceptthe