圆鼻巨蜥遗传多样性的RAPD分析（摘要）.pdfVIP

AgriculturalScienceTechnology，2011，12(3)：400—404 Copyright⑥2011，InformationInstituteofHAAS．Allrightsreserved AnimalScience RAPD AnalysisforGeneticDiversityof Varanussalvator FU Mei．1an’一 YUDong-rnei，WANGYing’，PENGJian-jun ． 1．LifeScienceandTechnologyInstitute，Ji’nanUniversity，Guangzhou510632；2 IdentificationCentreforWildlifeSpecies，SouthChina InstituteofEndangeredAnimals，Guangzhou510260 Abstract fObjective]TheaimwastocarryoutRAPDanalysisongeneticdiversityofVaranussalvator [Methodj2Orandomprimerswere usedforPCRamplificationofgenomicDNAof36individualsof salvator． 『Result]10primerscouldproducehighlyreproducibleRAPDbands． Atotalof2952DNA fragmentsweresuccessfullyamplified．Eachindividualgot82amplifiedbandsonaverage．47ofwhichshowedpolymor— phism．ThepolymorphicIocuspercentwas57．32％．Thegeneticdistanceamong36；ndividualsrangedfrOm 0．0359to0．3359withanaverage 0fO1359．TheNetsgenediversityindex(H)andShannon’sinformationindex(，)were0．1819and0．2630，respectively，indicatingthat salvaf0rhadgreatergeneticdiversity． 『Conclusion]ThephylogenetictreewasinferredbyusingUPGMAanalysis，itwasfoundthatthe36in- dividualscouldbeclassified asonegroup，andtherewasnoobviouspopulationdifferentiation． Keywords Varanussalvator；Geneticdiversity；RAPD；UPGMA analysis Varanussalvator．alsoknownascommonwatermonitor， thenasmallamountoftailtissuewasclipped．Afterethanol belongstoGenusVaranus，FamiIyVaranidae，Sauria，Squa— immersion．theyweresavedat一2O ℃ ． malaofReptilial1J．Itismainlydistributed inwestern，south— Methods westernandsoutherntropicalregionsofChina ．Foritsdell— DNA extraction 20 —30 matissueswere placed in1．5 ml ciousmeatwithhighnutritionaIvalue．indiscriminate over—ex— centrifugetube and cutwithscissors．Dneasytissue extrac- ploitation hasbeen carried outon itoveryears．Moreover． tion kitfQiagen Comp