肠道病毒71型和柯萨奇病毒A组16型实验室检测研究进展.pdfVIP

1878 2009 9 25 17 Occup andHealth ol. 25 No. 17 Sep 2009 肠道病毒 71型和柯萨奇病毒 A组 16型 = 实验室检测研究进展 Current Progress inM ethods for Laboratory Detection of Enterovirus 71 and CoxsackievirusA 16 金玉娟, 甘莉萍, 陈应坚,杨慧 JIN Yu-j uan, GAN L i-p ing, CHEN Ying-j ian, YANG H ui 71(Enterovirus, E 71) A 16( Coxsackievirus, CoxA 16) , , 2 2 , RT-PCR RT-PCR 肠道病毒 71型;柯萨奇病毒 A 组 16型; 实验室检测 : R37312 : A : 1004- 1257( 2009) 17- 1878- 03 Subject Research Progress on Laboratory Testing ofEnterovirus 71 and CoxsackievirusA 16 A uthors JIN Yu-juan, GAN L i-p ing, CH EN Ying-j ian, et a l1 ( Longgang D istrict Center f or D isease Control and P re en tion, Guangdong, 518172, China) A bstrac t Enterovirus 71( E 71) and Coxsackievirus A 16 ( CoxA 16) are known as major pathogens that cause hand-foot-and- mouth disease (HFMD), rapid and accurate diagnosis of these 2 pathogens is very mi portant for the treatment and control ofHFMD. This article reviewed the current research progress in laboratory detection of these 2 pathogens including virus isolation, serolog ical testing, M olecular biology methods ofRT-PCR, fluorescence RT-PCR, etc. K ey w ord s Enterovirus 71; CoxsackievirusA 16; Laboratory testing ( hand, foot andmouth disease, HFMD) Hep-2Hela (MK) ( Enterovirus, E ), ero, E 71 , 1957, , [ 3-4] 2006, () 4 , 2008 3, , , , , 2008 5 [ 1] , 2, , RNA, RNA , , ( 5~ 7 d), [ 5] 71 ( E 71) A 4591016