人EGFR显性负性突变体真核表达载体的构建、蛋白表达及亚细胞结构定位.pdfVIP

2266 堑四 医 学 (JFourthMilMedUniv)2009，3o(21) hitp：／／www．fmmuxb．cn · 研究原著 · 文章编号：1000-2790(2009)21-2266-05 人 EGFR显性负性突变体真核表达载体的构建、蛋 白表达及亚细胞结构 定位 廖 刚 ，mff-CZ，赵 林 ，张 能 ，汤为学。 (重庆医科大学：附属第一医院胃肠外科，。基础医学院病理生理学教研室，重庆400016) Construction ofthe eukaryotic vector DNA sequencing and bioinfomr aties analysis of nucleotide sequence． The expression ofDNEGFR—EGFP wasverified by carrying human dominant negative W esternBlot，anditspredominantlocalizationonthecellmen— epidermalgrowth factor receptor，its braheofCOS一7 cellswasidentified byLaserScanning Spectral expressionand sub．cellularlocalization ConfocalMicroscope．CONCLUSION ：Thesuccessfulconstruc— tionofpEGFPN1一DNEGFR，theexpressionandthesub—cellulra inCoS_7cells IocalizationofDNEGFR—EGFP inCOS-7cells．1aidasolidfoun— L1AO Gang ，WANG Zi—Wei，ZHAO Lin ，ZHANG Neng ，TANG dationforfurtherreserachon EGFR—trageteddominantnegative Wei—Xue strategy in cancergenetherapy． Department of Gastrointestinal Surged+， First Affiliated 【Keywords】epidemralgrowthfactorreceptor；dominantnegative Hospital，DepartmentofPathophysiology，BasicMedical College， mutant；singlenucleotidepolymorphism；directional ChongqingMedical University，Chongqing400016，China cloning；sub—cellularIcealization A【bstract】AIM：Toconstructtheeukaryoticexpressionvector