原创力文档Department of Chemical and Materials Engineering, Southern Taiwan University (南台科技大學化學工程與材料工程系).docVIP
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The Operating Conditions for Detection of Hydrogen Peroxide for the Carbon Paste Electrode Modified with Meldola’s Blue
Chi-Wen Lo (羅濟玟) Chung-Min Lien (連崇閔) Hau Lin (林浩)
Department of Chemical and Materials Engineering, Southern Taiwan University
(南台科技大學化學工程與材料工程系)
Nowadays because hydrogen peroxide is widely used in the industry, a rapid and convenient sensor for detecting the hydrogen peroxide has become an important research subject. In recent years, diabetes has become one of the top ten causes of death for the people in Taiwan. Therefore, developing a glucose biosensor which can detect the glucose rapidly and conveniently is also an important research subject. The glucose and oxygen can be catalyzed by the glucose oxidase to produce gluconic acid and hydrogen peroxide. The responding current of hydrogen peroxide is detected in the phosphate solution and then the concentration of the hydrogen peroxide can be obtained and consequently, the concentration of the glucose can be determined. A study was conducted to use the Meldola’s Blue to modify the carbon paste electrode. Because the Meldola’s Blue possesses the excellent catalytic characteristic, it can be used with the carbon powders which possess the excellent conductivity to make the carbon paste electrode and elevate the responding current of hydrogen peroxide. The TB (Time Base) graphs for different operating potentials, stirring rates, and pH values were plotted to determine the optimum operating conditions. At 30℃, -300 mV operating potential, 500 rpm stirring rate and in 0.05M phosphate solution ( pH = 7.4 ) , when the carbon paste electrode was modified with Meldola’s Blue [Meldola’s Blue : carbon powders : carbon paste = 2：15：15 ( weight ratio )] , the detection limit was 0.02 mM H2O2 , the linear range was 0.02～1.4 mM H2O2 , R2 = 0.9994 , and the sensitivity was 159.39 μA/cm2．mM H2O2 . For the unmodified carbon paste electrode, the detection limit was 0.02 mM H2O2 , the linear range was 0.02～1
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